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Molecular Pharmacology

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Research ArticleArticle

Low Molecular Weight Fucoidan and Heparin Enhance the Basic Fibroblast Growth Factor-Induced Tube Formation of Endothelial Cells through Heparan Sulfate-Dependent α6 Overexpression

Delphine Chabut, Anne-Marie Fischer, Sylvia Colliec-Jouault, Ingrid Laurendeau, Sabine Matou, Bernard Le Bonniec and Dominique Helley
Molecular Pharmacology September 2003, 64 (3) 696-702; DOI: https://doi.org/10.1124/mol.64.3.696
Delphine Chabut
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Anne-Marie Fischer
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Sylvia Colliec-Jouault
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Ingrid Laurendeau
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Sabine Matou
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Bernard Le Bonniec
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Dominique Helley
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Abstract

Basic fibroblast growth factor (FGF-2) activates its high-affinity receptors (FGFRs) but also acts through interaction with heparan sulfate proteoglycans (HSPG). Exogenous polysaccharides also modulate the angiogenic activity of FGF-2. We investigated the effect and mechanism of action of a low molecular weight fucoidan derivative (LMWF) on tube formation by human endothelial cells. LMWF has a better arterial antithrombotic potential in animals than low molecular weight heparin (LMWH). After stimulation of human umbilical vein endothelial cells (HUVEC) by FGF-2 and LMWF (or LMWH), we observed 1) using flow cytometry, an increase in the amount of the α6 integrin subunit; 2) using quantitative reverse transcription-polymerase chain reaction, an increase in α6 mRNA (higher with LMWF than with LMWH); and 3) using a Matrigel model, an increase in vascular tube formation (also higher with LMWF than with LMWH). A direct link between α6 overexpression and vascular tube formation was confirmed by use of an anti-α6 antibody: in its presence, there was no capillary network formation on Matrigel. Unexpectedly, an anti-FGFR blocking antibody had no effect on α6 over-expression, whereas stripping off the heparan sulfate with heparitinases abolished overexpression. Overall, our data suggest that FGF-2 stimulates α6 over-expression in HUVEC, through HSPG but independently from FGFR, and that LMWF (or LMWH) modulates this interaction. Expression of heparan sulfate proteoglycan increases after ischemic injury. Given its antithrombotic properties and its ability to potentiate tube formation of endothelial cells, LMWF may have to be considered for revascularization of ischemic areas.

  • Received February 27, 2003.
  • Accepted May 16, 2003.
  • The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 64 (3)
Molecular Pharmacology
Vol. 64, Issue 3
1 Sep 2003
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Research ArticleArticle

Low Molecular Weight Fucoidan and Heparin Enhance the Basic Fibroblast Growth Factor-Induced Tube Formation of Endothelial Cells through Heparan Sulfate-Dependent α6 Overexpression

Delphine Chabut, Anne-Marie Fischer, Sylvia Colliec-Jouault, Ingrid Laurendeau, Sabine Matou, Bernard Le Bonniec and Dominique Helley
Molecular Pharmacology September 1, 2003, 64 (3) 696-702; DOI: https://doi.org/10.1124/mol.64.3.696

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Research ArticleArticle

Low Molecular Weight Fucoidan and Heparin Enhance the Basic Fibroblast Growth Factor-Induced Tube Formation of Endothelial Cells through Heparan Sulfate-Dependent α6 Overexpression

Delphine Chabut, Anne-Marie Fischer, Sylvia Colliec-Jouault, Ingrid Laurendeau, Sabine Matou, Bernard Le Bonniec and Dominique Helley
Molecular Pharmacology September 1, 2003, 64 (3) 696-702; DOI: https://doi.org/10.1124/mol.64.3.696
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