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Molecular Pharmacology

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Research ArticleArticle

Inhibition of Interleukin-4 Production in CD4+ T Cells by Peroxisome Proliferator-Activated Receptor-γ (PPAR-γ) Ligands: Involvement of Physical Association between PPAR-γ and the Nuclear Factor of Activated T Cells Transcription Factor

Su Wol Chung, Bok Yun Kang and Tae Sung Kim
Molecular Pharmacology November 2003, 64 (5) 1169-1179; DOI: https://doi.org/10.1124/mol.64.5.1169
Su Wol Chung
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Bok Yun Kang
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Tae Sung Kim
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Abstract

Peroxisome proliferator-activated receptor-γ (PPAR-γ) has been implicated in the regulation of multiple inflammatory processes. However, little is known of PPAR-γ in the regulation of interleukin (IL)-4 expression in T cells. In this study, the effects of PPAR-γ ligands on production of IL-4, a pro-inflammatory cytokine associated with the pathophysiology of allergic diseases, were investigated. 15-Deoxy-Δ12,14 prostaglandin J2 (15d-PGJ2) and ciglitazone, two representative PPAR-γ ligands, significantly inhibited IL-4 production in both antigen-stimulated primary CD4+ T cells and the phorbol 12-myristate 13-acetate (PMA)/ionomycin-activated EL-4 T cell line. 15d-PGJ2 and ciglitazone inhibited the activation of IL-4 gene promoter in EL-4 T cells transiently transfected with IL-4 promoter/reporter constructs, and the repressive effect mapped to a region in the IL-4 promoter containing binding sites for nuclear factor of activated T cells (NF-AT). The activation of T cells by PMA/ionomycin resulted in a marked enhancement of the binding activities to the NF-AT site that was significantly inhibited by the addition of PPAR-γ ligands. In cotransfected EL-4 T cells, PPAR-γ also inhibited the activation of the IL-4 promoter at multiple NF-AT sites in a ligand-dependent manner. NF-ATc1 bound PPAR-γ both in vivo and in vitro, and the interaction interfaces involved the Rel similarity domain of NF-ATc1. In cotransfections of HeLa cells, PPAR-γ inhibited the NF-ATc1 transactivation in a ligand-dependent manner. Coexpression of p300 or AP-1 relieved the PPAR-γ ligand-mediated inhibition of the NF-AT transactivation. From these results, we propose that PPAR-γ ligand-mediated suppression of IL-4 production in CD4+ T cells may involve both inhibition of the NFAT-DNA interactions and competitive recruitment of transcription integrators between NF-AT and PPAR-γ.

  • Received April 23, 2003.
  • Accepted August 5, 2003.
  • The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 64 (5)
Molecular Pharmacology
Vol. 64, Issue 5
1 Nov 2003
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Research ArticleArticle

Inhibition of Interleukin-4 Production in CD4+ T Cells by Peroxisome Proliferator-Activated Receptor-γ (PPAR-γ) Ligands: Involvement of Physical Association between PPAR-γ and the Nuclear Factor of Activated T Cells Transcription Factor

Su Wol Chung, Bok Yun Kang and Tae Sung Kim
Molecular Pharmacology November 1, 2003, 64 (5) 1169-1179; DOI: https://doi.org/10.1124/mol.64.5.1169

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Research ArticleArticle

Inhibition of Interleukin-4 Production in CD4+ T Cells by Peroxisome Proliferator-Activated Receptor-γ (PPAR-γ) Ligands: Involvement of Physical Association between PPAR-γ and the Nuclear Factor of Activated T Cells Transcription Factor

Su Wol Chung, Bok Yun Kang and Tae Sung Kim
Molecular Pharmacology November 1, 2003, 64 (5) 1169-1179; DOI: https://doi.org/10.1124/mol.64.5.1169
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