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Research ArticleArticle

Evidence of a Functional Role for p21WAF1/CIP1 Down-Regulation in Synergistic Antileukemic Interactions between the Histone Deacetylase Inhibitor Sodium Butyrate and Flavopiridol

Roberto R. Rosato, Jorge A. Almenara, Chunrong Yu and Steven Grant
Molecular Pharmacology March 2004, 65 (3) 571-581; DOI: https://doi.org/10.1124/mol.65.3.571
Roberto R. Rosato
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Jorge A. Almenara
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Chunrong Yu
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Steven Grant
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Abstract

The functional significance of disruption of p21WAF1/CIP1 induction by flavopiridol (FP) in human leukemia cells (Jurkat) exposed to the histone deacetylase (HDAC) inhibitor sodium butyrate (SB) was investigated. Coexposure of leukemic cells to FP blocked SB-mediated induction of p21WAF1/CIP1 and resulted in a marked increase in mitochondrial injury, activation of procaspases-3 and -8, Bid cleavage, and PARP degradation. Enforced expression of p21WAF1/CIP1 (i.e., in Jurkat cells inducibly expressing p21WAF1/CIP1 under the control of a doxycycline-responsive promoter) partially but significantly reduced cytochrome c and apoptosis-inducing factor release, loss of mitochondrial membrane potential, caspase-3 and -8 activation, Bid cleavage, poly(ADP-ribose)polymerase (PARP) degradation, and apoptosis in response to SB/FP. Furthermore, increasing expression of p21WAF1/CIP1 (i.e., by culturing cells in the presence of higher concentrations of doxycycline) rendered cells more resistant to SB/FP-mediated lethality. Enforced expression of p21WAF1/CIP1 did not modify SB/FP-mediated JNK activation or generation of reactive oxygen species. Consistent with these results, Jurkat cells stably expressing a p21WAF1/CIP1 nuclear localization mutant (p21ΔNLS) were also resistant to SB/FP-mediated mitochondrial injury, activation of procaspases-3 and -8, PARP cleavage, and apoptosis. Finally, enforced expression of full-length or ectopic expression of ΔNLS p21WAF1/CIP1 increased the amount of p21WAF1/CIP1 coimmunoprecipitating with procaspase-3. Together, these findings suggest that interruption of HDAC-mediated p21WAF1/CIP1 induction by FP plays a significant functional role in potentiating apoptosis, possibly by preventing the formation of a procaspase-3/p21WAF1/CIP1 complex.

  • Received July 30, 2003.
  • Accepted December 4, 2003.
  • The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 65 (3)
Molecular Pharmacology
Vol. 65, Issue 3
1 Mar 2004
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Research ArticleArticle

Evidence of a Functional Role for p21WAF1/CIP1 Down-Regulation in Synergistic Antileukemic Interactions between the Histone Deacetylase Inhibitor Sodium Butyrate and Flavopiridol

Roberto R. Rosato, Jorge A. Almenara, Chunrong Yu and Steven Grant
Molecular Pharmacology March 1, 2004, 65 (3) 571-581; DOI: https://doi.org/10.1124/mol.65.3.571

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Research ArticleArticle

Evidence of a Functional Role for p21WAF1/CIP1 Down-Regulation in Synergistic Antileukemic Interactions between the Histone Deacetylase Inhibitor Sodium Butyrate and Flavopiridol

Roberto R. Rosato, Jorge A. Almenara, Chunrong Yu and Steven Grant
Molecular Pharmacology March 1, 2004, 65 (3) 571-581; DOI: https://doi.org/10.1124/mol.65.3.571
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