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Research ArticleArticle

Enhancement of Cardiac L-Type Ca2+ Currents in Transgenic Mice with Cardiac-Specific Overexpression of CYP2J2

Yong-Fu Xiao, Qingen Ke, John M. Seubert, J. Alyce Bradbury, Joan Graves, Laura M. DeGraff, John R. Falck, Kris Krausz, Harry V. Gelboin, James P. Morgan and Darryl C. Zeldin
Molecular Pharmacology December 2004, 66 (6) 1607-1616; DOI: https://doi.org/10.1124/mol.104.004150
Yong-Fu Xiao
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Qingen Ke
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John M. Seubert
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J. Alyce Bradbury
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Joan Graves
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Laura M. DeGraff
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John R. Falck
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Kris Krausz
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Harry V. Gelboin
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James P. Morgan
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Darryl C. Zeldin
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Abstract

CYP2J2 is abundant in cardiomyocytes and is involved in the metabolism of arachidonic acid (AA) to epoxyeicosatrienoic acids (EETs), which affect multiple cell functions. In this study, we investigated the effect of overexpression of CYP2J2 on cardiac L-type Ca2+ currents (ICa) in adult transgenic mice. Cardiac-specific overexpression of CYP2J2 was achieved using the α-myosin heavy chain promoter. ICa was recorded from isolated ventricular cardiomyocytes. Compared with the wild-type cardiomyocytes (n = 60), the density of ICa was significantly increased by 40 ± 9% in the CYP2J2 transgenic cardiomyocytes (n = 71; P < 0.001). N-Methylsulfonyl-6-(2-proparglyloxyphenyl)hexanamide (MS-PPOH), a specific inhibitor of EET biosynthesis, and clotrimazole, a cytochrome P450 inhibitor, significantly reduced ICa in both wild-type and transgenic cardiomyocytes; however, MS-PPOH inhibited ICa to a greater extent in the CYP2J2 transgenic cells (n = 10) than in the wild-type cells (n = 10; P < 0.01). Addition of 11,12-EET significantly restored ICa in MS-PPOH-treated cells. Intracellular dialysis with either of two inhibitory monoclonal antibodies against CYP2J2 significantly reduced ICa in both wild-type and transgenic mice. Membrane-permeable 8-bromo-cAMP and the β-adrenergic agonist isoproterenol significantly reversed the monoclonal antibody-induced inhibition of ICa. In addition, the total protein level of the α1 subunit of the Cav1.2 L-type Ca2+ channel was not altered in CYP2J2 transgenic hearts, but the phosphorylated portion was markedly increased. In conclusion, overexpression of CYP2J2 increases ICa in CYP2J2 transgenic cardiomyocytes via a mechanism that involves cAMP-protein kinase A-dependent phosphorylation of the L-type Ca2+ channel.

  • Received June 23, 2004.
  • Accepted September 10, 2004.
  • The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 66 (6)
Molecular Pharmacology
Vol. 66, Issue 6
1 Dec 2004
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Research ArticleArticle

Enhancement of Cardiac L-Type Ca2+ Currents in Transgenic Mice with Cardiac-Specific Overexpression of CYP2J2

Yong-Fu Xiao, Qingen Ke, John M. Seubert, J. Alyce Bradbury, Joan Graves, Laura M. DeGraff, John R. Falck, Kris Krausz, Harry V. Gelboin, James P. Morgan and Darryl C. Zeldin
Molecular Pharmacology December 1, 2004, 66 (6) 1607-1616; DOI: https://doi.org/10.1124/mol.104.004150

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Research ArticleArticle

Enhancement of Cardiac L-Type Ca2+ Currents in Transgenic Mice with Cardiac-Specific Overexpression of CYP2J2

Yong-Fu Xiao, Qingen Ke, John M. Seubert, J. Alyce Bradbury, Joan Graves, Laura M. DeGraff, John R. Falck, Kris Krausz, Harry V. Gelboin, James P. Morgan and Darryl C. Zeldin
Molecular Pharmacology December 1, 2004, 66 (6) 1607-1616; DOI: https://doi.org/10.1124/mol.104.004150
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