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Research ArticleArticle

DNA Methylation-Related Chromatin Modification in the Regulation of Mouse δ-Opioid Receptor Gene

Guilin Wang, Tiancheng Liu, Li-Na Wei, Ping-Yee Law and Horace H. Loh
Molecular Pharmacology June 2005, 67 (6) 2032-2039; DOI: https://doi.org/10.1124/mol.105.011056
Guilin Wang
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Tiancheng Liu
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Li-Na Wei
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Ping-Yee Law
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Horace H. Loh
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Abstract

DNA methylation plays critical roles in gene-silencing through chromatin modification. We reported previously that promoter-region CpG methylation repressed mouse δ-opioid receptor (mDOR) gene expression. In the current study, we demonstrated that the methylation of mDOR gene promoter is correlated with a repressive chromatin structure that has less HaeIII and MspI nuclear accessibility and more deacetylated histone H3 and H4 than that of unmethylated mDOR promoter. Chromatin immunoprecipitation analysis showed the association of a methyl-CpG-binding domain protein 2 (MBD2) with methylated mDOR promoter. Transient expression of MBD2 enhanced the repression of partially methylated mDOR promoter activity, and this repression was partially reversed by treatment of trichostatin A, a specific histone deacetylase inhibitor, indicating that MBD2 may mediate DNA methylation-related chromatin modification through recruiting histone deacetylases to mDOR promoter region. In addition, trichostatin A treatment increased both methylated mDOR promoter activity in a transient transfection assay and endogenous mDOR mRNA level in Neuro2A cells. Taken together, these results demonstrate that the mDOR gene expression is regulated by DNA methylation-related chromatin modification, especially histone acetylation and deacetylation.

  • Received January 10, 2005.
  • Accepted March 14, 2005.
  • The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 67 (6)
Molecular Pharmacology
Vol. 67, Issue 6
1 Jun 2005
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Research ArticleArticle

DNA Methylation-Related Chromatin Modification in the Regulation of Mouse δ-Opioid Receptor Gene

Guilin Wang, Tiancheng Liu, Li-Na Wei, Ping-Yee Law and Horace H. Loh
Molecular Pharmacology June 1, 2005, 67 (6) 2032-2039; DOI: https://doi.org/10.1124/mol.105.011056

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Research ArticleArticle

DNA Methylation-Related Chromatin Modification in the Regulation of Mouse δ-Opioid Receptor Gene

Guilin Wang, Tiancheng Liu, Li-Na Wei, Ping-Yee Law and Horace H. Loh
Molecular Pharmacology June 1, 2005, 67 (6) 2032-2039; DOI: https://doi.org/10.1124/mol.105.011056
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