Fig. 1.
Immunochemistry using antibodies against CaV3.1 (column 1) and CaV3.2 (column 2). A and B, CaV3.1-transfected HEK-293T cells. C and D, CaV3.2-transfected HEK-293T cells. Cell nuclei are blue (Hoechst staining); tetramethylrhodamine isothiocyanate-labeled secondary antibodies were used (red staining). E and F, LVA staining in organotypic hippocampal slices (fluorescein isothiocyanate-labeled secondary antibodies). CA3, CA3 pyramidal cell layer; DG, dentate gyrus. Inset, higher magnification in CA1 pyramidal layer. Scale bars: A–D, 10 μm; E and F, 100 μm; inset, 20 μm.