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Molecular Pharmacology

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Research ArticleArticle

Mouse β-TC6 Insulinoma Cells: High Expression of Functional α3β4 Nicotinic Receptors Mediating Membrane Potential, Intracellular Calcium, and Insulin Release

Masahiro Ohtani, Takami Oka, Maryna Badyuk, Yingxian Xiao, Kenneth J. Kellar and John W. Daly
Molecular Pharmacology March 2006, 69 (3) 899-907; DOI: https://doi.org/10.1124/mol.105.014902
Masahiro Ohtani
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Takami Oka
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Maryna Badyuk
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Yingxian Xiao
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Kenneth J. Kellar
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John W. Daly
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Abstract

Nicotine elicited membrane depolarization, elevation of intracellular calcium, rubidium efflux, and release of insulin from mouse β-TC6 insulinoma cells. Such responses were blocked by the nicotinic antagonist mecamylamine but not by the muscarinic antagonist atropine. Neither the selective α4β2 antagonist dihydro-β-erythroidine nor the selective α7 antagonist methyllycaconitine significantly blocked the nicotine-elicited depolarization or the calcium response. The elevation of intracellular calcium did not occur in calcium-free media, indicating that the increase in intracellular calcium was due to the influx of calcium. The rank order of potency for nicotinic agonists was as follows: epibatidine > nicotine = 3-(azetidinylmethoxy)pyridine (A-85380), cytisine, dimethylphenylpiperazinium (DMPP). Cytisine and DMPP seemed to be partial agonists. The density of nicotinic receptors measured by [3H]epibatidine binding was 7-fold higher in membranes from β-TC6 cells than in rat brain membranes. No binding of 125I-A-85380 was detected, indicating the absence of β2-containing receptors. Reverse transcription-polymerase chain reaction analyses indicated the presence of mRNA for α3 and α4 subunits and β2 and β4 subunits in β-TC6 cells. The binding and functional data suggest that the major nicotinic receptor is composed of α3 and β4 subunits. The β-TC6 cells thus provide a model system for pharmacological study of such nicotinic receptors.

  • Received May 18, 2005.
  • Accepted December 6, 2005.
  • The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 69 (3)
Molecular Pharmacology
Vol. 69, Issue 3
1 Mar 2006
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Research ArticleArticle

Mouse β-TC6 Insulinoma Cells: High Expression of Functional α3β4 Nicotinic Receptors Mediating Membrane Potential, Intracellular Calcium, and Insulin Release

Masahiro Ohtani, Takami Oka, Maryna Badyuk, Yingxian Xiao, Kenneth J. Kellar and John W. Daly
Molecular Pharmacology March 1, 2006, 69 (3) 899-907; DOI: https://doi.org/10.1124/mol.105.014902

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Research ArticleArticle

Mouse β-TC6 Insulinoma Cells: High Expression of Functional α3β4 Nicotinic Receptors Mediating Membrane Potential, Intracellular Calcium, and Insulin Release

Masahiro Ohtani, Takami Oka, Maryna Badyuk, Yingxian Xiao, Kenneth J. Kellar and John W. Daly
Molecular Pharmacology March 1, 2006, 69 (3) 899-907; DOI: https://doi.org/10.1124/mol.105.014902
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