Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

Short- and Long-Term Regulation of Adenylyl Cyclase Activity by δ-Opioid Receptor Are Mediated by Gαi2 in Neuroblastoma N2A Cells

Lei Zhang, Joan Tetrault, Wei Wang, Horace H. Loh and Ping-Yee Law
Molecular Pharmacology June 2006, 69 (6) 1810-1819; DOI: https://doi.org/10.1124/mol.105.021352
Lei Zhang
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joan Tetrault
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wei Wang
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Horace H. Loh
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ping-Yee Law
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Activation of the opioid receptor results in short-term inhibition of intracellular cAMP levels followed by receptor desensitization and subsequent increase of cAMP above the control level (adenylyl cyclase superactivation). Using adenovirus to deliver pertussis toxin-insensitive mutants of the α-subunits of Gi/o that are expressed in neuroblastoma Neuro2A cells (Gαi2, Gαi3, and Gαo), we examined the identities of the G proteins involved in the short- and long-term action of the δ-opioid receptor (DOR). Pertussis toxin pretreatment completely abolished the ability of [d-Pen2, d-Pen5]-enkephalin (DPDPE) to inhibit forskolin-stimulated intracellular cAMP production. Expression of the C352L mutant of Gαi2, and not the C351L mutants of Gαi3 or Gαo, rescued the short-term effect of DPDPE after pertussis toxin treatment. The ability of Gαi2 in mediating DOR inhibition of adenylyl cyclase activity was also reflected in the ability of Gαi2, not Gαi3 or Gαo, to coimmunoprecipitate with DOR. Coincidently, after long-term DPDPE treatment, pertussis toxin treatment eliminated the antagonist naloxone-induced superactivation of adenylyl cyclase activity. Again, only the C352L mutant of Gαi2 restored the adenylyl cyclase superactivation after pertussis toxin treatment. More importantly, the C352L mutant of Gαi2 remained associated with DOR after long-term agonist and pertussis toxin treatment whereas the wild-type Gαi2 did not. These data suggest that Gαi2 serves as the signaling molecule in both DOR-mediated short- and long-term regulation of adenylyl cyclase activity.

  • Received November 30, 2005.
  • Accepted March 8, 2006.
  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology: 69 (6)
Molecular Pharmacology
Vol. 69, Issue 6
1 Jun 2006
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Short- and Long-Term Regulation of Adenylyl Cyclase Activity by δ-Opioid Receptor Are Mediated by Gαi2 in Neuroblastoma N2A Cells
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

Short- and Long-Term Regulation of Adenylyl Cyclase Activity by δ-Opioid Receptor Are Mediated by Gαi2 in Neuroblastoma N2A Cells

Lei Zhang, Joan Tetrault, Wei Wang, Horace H. Loh and Ping-Yee Law
Molecular Pharmacology June 1, 2006, 69 (6) 1810-1819; DOI: https://doi.org/10.1124/mol.105.021352

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

Short- and Long-Term Regulation of Adenylyl Cyclase Activity by δ-Opioid Receptor Are Mediated by Gαi2 in Neuroblastoma N2A Cells

Lei Zhang, Joan Tetrault, Wei Wang, Horace H. Loh and Ping-Yee Law
Molecular Pharmacology June 1, 2006, 69 (6) 1810-1819; DOI: https://doi.org/10.1124/mol.105.021352
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Therapeutic Effects of FGF23 Antagonists in Hyp Mice
  • TRPV3 and TRPV4 Channels Coassemble into Heterotetramers
  • Secretin Amino-Terminal Structure-Activity Relationships and Complementary Mutagenesis at the Site of Docking to the Secretin Receptor
Show more Article

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2022 by the American Society for Pharmacology and Experimental Therapeutics