Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

Adenosine Kinase of Sarcoma 180 Cells

N6-Substituted Adenosines as Substrates and Inhibitors

A. Y. DIVEKAR and M. T. HAKALA
Molecular Pharmacology November 1971, 7 (6) 663-673;
A. Y. DIVEKAR
Department of Experimental Therapeutics, Roswell Park Memorial Institute, New York State Department of Health, Buffalo, New York 14203
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M. T. HAKALA
Department of Experimental Therapeutics, Roswell Park Memorial Institute, New York State Department of Health, Buffalo, New York 14203
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Adenosine kinase (ATP:adenosine 5'-phosphotransferase, EC 2.7.1.20) was partially purified by DEAE-cellulose column chromatography from Sarcoma 180 cells grown in vitro. This enzyme preparation, which was free of adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4) but contained adenylate kinase (ATP:AMP phosphotransferase, EC 2.7.4.3), was studied with respect to its kinetic properties and specificity for substrates and inhibitors.

At pH 7.0 and 35° the Km for adenosine was 0.5 µM and the Vmax was 63 mµmoles/mg of protein per minute. Strong substrate inhibition was observed at adenosine concentrations greater than 4 µM, 50% inhibition occurring at about 100 µM. The reaction required ATP (Km = 200 µM) and Mg++. The optimal Mg++ concentrations were 0.1 and 0.25 mM at 0.5 and 2.5 mM ATP, respectively. Concentrations of Mg++ higher than these were inhibitory, and Mn++ substituted poorly for Mg++.

Most of the N6-substituted adenosine analogues which were substrates of adenosine kinase inhibited the growth of S-180 cells in vitro. Among these were two compounds which are also known to be potent cytokinins in plant systems, namely, N6-furfuryl- and N6-(Δ2-isopentenyl)adenosine. The 5'-monophosphate of the latter compound was not phosphorylated further by adenylate kinase. The N6-substituted adenosines which were poorly or not at all phosphorylated by adenosine kinase were also poor inhibitors of S-180 cells in vitro. Several of these were potent inhibitors of the kinase, such as N6-phenyladenosine, which had a Ki value of 0.6 µM.

ACKNOWLEDGMENT The authors wish to express their appreciation to Miss Dorris Sugg for her excellent assistance in these studies.

  • Copyright ©, 1971, by Academic Press, Inc.

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology
Vol. 7, Issue 6
1 Nov 1971
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Editorial Board (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Adenosine Kinase of Sarcoma 180 Cells
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

Adenosine Kinase of Sarcoma 180 Cells

A. Y. DIVEKAR and M. T. HAKALA
Molecular Pharmacology November 1, 1971, 7 (6) 663-673;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

Adenosine Kinase of Sarcoma 180 Cells

A. Y. DIVEKAR and M. T. HAKALA
Molecular Pharmacology November 1, 1971, 7 (6) 663-673;
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • The binding site for KCI807 in the androgen receptor
  • Fatty acid amide hydrolase in cisplatin nephrotoxicity
  • eCB Signaling System in hiPSC-Derived Neuronal Cultures
Show more Article

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics