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Molecular Pharmacology

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Research ArticleArticle

Interaction of Cytochrome P-450 with Antibodies Raised against a Solubilized P-450 Preparation from Hepatic Microsomes

F. MITANI, R. J. WINCHESTER, A. P. ALVARES, A. P. POLAND and A. KAPPAS
Molecular Pharmacology November 1971, 7 (6) 689-696;
F. MITANI
The Rockefeller University, New York, New York 10021
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R. J. WINCHESTER
The Rockefeller University, New York, New York 10021
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A. P. ALVARES
The Rockefeller University, New York, New York 10021
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A. P. POLAND
The Rockefeller University, New York, New York 10021
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A. KAPPAS
The Rockefeller University, New York, New York 10021
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Abstract

Soluble cytochrome P-450 prepared from microsomes of rat liver or from livers of chick embryos was shown to be capable of eliciting an immunological response in rabbits. The γ-globulin fraction from sera of immunized rabbits reacted with soluble P-450 of the same species. Precipitating reactions were obtained when antibody and antigen were incubated in the fluid phase, as well as by agar diffusion techniques. Lack of cross-reactivity between species was demonstrated when antibody to rat cytochrome P-450 was allowed to react with soluble P-450 from chick embryo livers and when antibody to chick cytochrome P-450 was allowed to react with soluble P-450 from rat liver. The Precipitate obtained upon reaction of antibody and antigen from the same species showed a typical CO difference spectrum with an absorption maximum at 450 nm and a type II difference spectrum with aniline. However, no type I difference spectrum could be obtained after the addition of sodium hexobarbital to the antibody-antigen complex, suggesting that the binding site of the immune complex may be at or near the type I binding site.

  • Copyright ©, 1971, by Academic Press, Inc.

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Molecular Pharmacology
Vol. 7, Issue 6
1 Nov 1971
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Research ArticleArticle

Interaction of Cytochrome P-450 with Antibodies Raised against a Solubilized P-450 Preparation from Hepatic Microsomes

F. MITANI, R. J. WINCHESTER, A. P. ALVARES, A. P. POLAND and A. KAPPAS
Molecular Pharmacology November 1, 1971, 7 (6) 689-696;

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Research ArticleArticle

Interaction of Cytochrome P-450 with Antibodies Raised against a Solubilized P-450 Preparation from Hepatic Microsomes

F. MITANI, R. J. WINCHESTER, A. P. ALVARES, A. P. POLAND and A. KAPPAS
Molecular Pharmacology November 1, 1971, 7 (6) 689-696;
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