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Molecular Pharmacology

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Research ArticleArticle

Apigenin Inhibits Immunostimulatory Function of Dendritic Cells: Implication of Immunotherapeutic Adjuvant

Man-Soo Yoon, Jun Sik Lee, Byoung-Moon Choi, Young-Il Jeong, Chang-Min Lee, Jong-Hoon Park, Yuseok Moon, Si-Chan Sung, Sang Kwon Lee, Yun Hee Chang, Hae Young Chung and Yeong-Min Park
Molecular Pharmacology September 2006, 70 (3) 1033-1044; DOI: https://doi.org/10.1124/mol.106.024547
Man-Soo Yoon
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Jun Sik Lee
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Byoung-Moon Choi
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Young-Il Jeong
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Chang-Min Lee
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Jong-Hoon Park
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Yuseok Moon
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Si-Chan Sung
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Sang Kwon Lee
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Yun Hee Chang
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Hae Young Chung
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Yeong-Min Park
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Abstract

Apigenin, one of the most common flavonoids, has been shown to possess anti-inflammatory, anticarcinogenic, and free radical-scavenging properties. However, the influence of apigenin on the immunostimulatory effects and maturation of dendritic cells (DC) remains, for the most part, unknown. In this study, we have attempted to ascertain whether apigenin influences the expression of surface molecules, dextran uptake, cytokine production, and T-cell differentiation as well as the signaling pathways underlying these phenomena in murine bone marrow-derived DC. In the presence of apigenin, CD80, CD86, and major histocompatibility complex class I and II molecules, expressions on DC were significantly suppressed, and lipopolysaccharide (LPS)-induced interleukin (IL)-12 expression was impaired. The DC proved highly efficient at antigen capture, as evidenced by the observation of mannose receptor-mediated endocytosis in the presence of apigenin. The LPS-induced activation of mitogen-activated protein kinase, the nuclear translocation of its nuclear factor-κB p65 subunit, and the induction of the T-helper 1 response were all impaired in the presence of apigenin, whereas the cell-mediated immune response remained normal. These findings provide new insight into the immunopharmacological functions of apigenin and its effects on DC, and they may also prove useful in the development of adjuvant therapies for individuals suffering from acute or chronic DC-associated diseases.

Footnotes

  • This work was supported by the Korea Science and Engineering Foundation through National Research Laboratory Program grant M105-0000000805J000000810 and Medical Research Institute grant 2005-22, Pusan National University.

  • M.-S.Y., J.S.L., and B.-M.C. contributed equally to this work.

  • ABBREVIATIONS: DC, dendritic cell(s); MHC, major histocompatibility complex; TNF, tumor necrosis factor; LPS, lipopolysaccharide; IL, interleukin; NF-κB, nuclear factor-κB; MAPK, mitogen-activated protein kinase; BM, bone marrow; BM-DC, bone marrow-dendritic cells; ERK, extracellular signal-regulated kinase; JNK, c-Jun NH2-terminal kinase; CHS, contact hypersensitivity; rm, recombinant mouse; ELISA, enzyme-linked immunosorbent assay; IFN, interferon; FITC, fluorescein isothiocyanate; PE, phycoerythrin; mAb, monoclonal antibody; Ab, antibody; FBS, fetal bovine serum; DMSO, dimethyl sulfoxide; PBS, phosphate-buffered saline; FACS, fluorescence-activated cell sorter; CFSE, 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester; p-, phospho-; TNBS, 2,4,6-trinitrobenzenesulfonic acid; TNCB, 2,4,6-trinitrochlorobenzene; Th, T-helper; TNBS-DC, 2,4,6-trinitrobenzenesulfonic acid-dendritic cell; MFI, mean fluorescence intensity; 2-ME, 2-mercaptoethanol.

    • Received March 15, 2006.
    • Accepted June 16, 2006.
  • The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 70 (3)
Molecular Pharmacology
Vol. 70, Issue 3
1 Sep 2006
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Research ArticleArticle

Apigenin Inhibits Immunostimulatory Function of Dendritic Cells: Implication of Immunotherapeutic Adjuvant

Man-Soo Yoon, Jun Sik Lee, Byoung-Moon Choi, Young-Il Jeong, Chang-Min Lee, Jong-Hoon Park, Yuseok Moon, Si-Chan Sung, Sang Kwon Lee, Yun Hee Chang, Hae Young Chung and Yeong-Min Park
Molecular Pharmacology September 1, 2006, 70 (3) 1033-1044; DOI: https://doi.org/10.1124/mol.106.024547

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Research ArticleArticle

Apigenin Inhibits Immunostimulatory Function of Dendritic Cells: Implication of Immunotherapeutic Adjuvant

Man-Soo Yoon, Jun Sik Lee, Byoung-Moon Choi, Young-Il Jeong, Chang-Min Lee, Jong-Hoon Park, Yuseok Moon, Si-Chan Sung, Sang Kwon Lee, Yun Hee Chang, Hae Young Chung and Yeong-Min Park
Molecular Pharmacology September 1, 2006, 70 (3) 1033-1044; DOI: https://doi.org/10.1124/mol.106.024547
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