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Research ArticleArticle

The Antiestrogen Tamoxifen Activates BK Channels and Stimulates Proliferation of MCF-7 Breast Cancer Cells

Guyllaume Coiret, Anne-Sophie Borowiec, Pascal Mariot, Halima Ouadid-Ahidouch and Fabrice Matifat
Molecular Pharmacology March 2007, 71 (3) 843-851; DOI: https://doi.org/10.1124/mol.106.028290
Guyllaume Coiret
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Anne-Sophie Borowiec
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Pascal Mariot
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Halima Ouadid-Ahidouch
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Fabrice Matifat
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Abstract

In the present study, we investigated the effect of the antiestrogen compound tamoxifen on BK channels by the use of the patch-clamp technique. The perfusion of 10 nM tamoxifen significantly increased the magnitude of a voltage-dependent K+ current by 22.6 ± 10.6% (n = 23). The effect of tamoxifen was always obtained in the first minute, peaked at 5.9 ± 2.2 min (n = 23), and was abolished by the perfusion of tetraethylammonium (0.5 mM), charybdotoxin (50 nM), or iberiotoxin (100 nM). The stimulatory effect of 10 nM tamoxifen was the same at low (50 nM) and high (700 nM) internal calcium concentration and was not additive to that of 17-β-estradiol (E2) or its membrane-impermeant form, β-estradiol 6-(O-carboxymethyl)oxime:bovine serum albumin. Furthermore, the effect of tamoxifen was still recorded in the presence of the selective estrogen receptor antagonist faslodex (ICI-182,780; 1 μM). At the single-channel level, tamoxifen significantly increased the open probability of the BK channel by 46.2 ± 10.1% (n = 4) without changing its unitary conductance. Moreover, we show here that the stimulation of BK channel activity by tamoxifen is involved in MCF-7 cell proliferation. Taken together, these results permitted us to identify the BK channel as the molecular target of tamoxifen that probably acts at the same extracellular molecular level as E2. The site of action of tamoxifen is probably the channel itself or the auxiliary β subunits.

Footnotes

  • This work was supported by La Ligue Contre Le Cancer, La Région Picardie, the Ministère de l'Education Nationale de l'Enseignement Supérieur et de la Recherche, and l'Association de la Recherche contre le Cancer (France). A.-S.B. was supported financially by the Ministère de l'Education Nationale de l'Enseignement Supérieur et de la Recherche.

  • Article, publication date, and citation information can be found at http://molpharm.aspetjournals.org.

  • doi:10.1124/mol.106.028290.

  • ABBREVIATIONS: E2, 17-β-estradiol; FCS, fetal calf serum; DCCFCS, dextran-coated charcoal-treated fetal calf serum; IbTx, iberiotoxin; ChTx, charybdotoxin; ER, estrogen receptor; NPo, open probability; E2BSA, β-estradiol 6-(O-carboxymethyl)oxime:bovine serum albumin; SMC, smooth muscle cells; EMEM, Eagle's minimal essential medium; BAPTA, 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid; TEA, tetraethylammonium; PCR, polymerase chain reaction; PBS, phosphate-buffered saline; ANOVA, analysis of variance; ICI-182,780, faslodex.

    • Received June 28, 2006.
    • Accepted December 12, 2006.
  • The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 71 (3)
Molecular Pharmacology
Vol. 71, Issue 3
1 Mar 2007
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Research ArticleArticle

The Antiestrogen Tamoxifen Activates BK Channels and Stimulates Proliferation of MCF-7 Breast Cancer Cells

Guyllaume Coiret, Anne-Sophie Borowiec, Pascal Mariot, Halima Ouadid-Ahidouch and Fabrice Matifat
Molecular Pharmacology March 1, 2007, 71 (3) 843-851; DOI: https://doi.org/10.1124/mol.106.028290

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Research ArticleArticle

The Antiestrogen Tamoxifen Activates BK Channels and Stimulates Proliferation of MCF-7 Breast Cancer Cells

Guyllaume Coiret, Anne-Sophie Borowiec, Pascal Mariot, Halima Ouadid-Ahidouch and Fabrice Matifat
Molecular Pharmacology March 1, 2007, 71 (3) 843-851; DOI: https://doi.org/10.1124/mol.106.028290
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