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Research ArticleArticle

Inhibition of Sodium Channel Gating by Trapping the Domain II Voltage Sensor with Protoxin II

Stanislav Sokolov, Richard L. Kraus, Todd Scheuer and William A. Catterall
Molecular Pharmacology March 2008, 73 (3) 1020-1028; DOI: https://doi.org/10.1124/mol.107.041046
Stanislav Sokolov
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Richard L. Kraus
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Todd Scheuer
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William A. Catterall
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Abstract

ProTx-II, an inhibitory cysteine knot toxin from the tarantula Thrixopelma pruriens, inhibits voltage-gated sodium channels. Using the cut-open oocyte preparation for electrophysiological recording, we show here that ProTx-II impedes movement of the gating charges of the sodium channel voltage sensors and reduces maximum activation of sodium conductance. At a concentration of 1 μM, the toxin inhibits 65.3 ± 4.1% of the sodium conductance and 24.6 ± 6.8% of the gating current of brain Nav1.2a channels, with a specific effect on rapidly moving gating charge. Strong positive prepulses can reverse the inhibitory effect of ProTx-II, indicating voltage-dependent dissociation of the toxin. Voltage-dependent reversal of the ProTx-II effect is more rapid for cardiac Nav1.5 channels, suggesting subtype-specific action of this toxin. Voltage-dependent binding and block of gating current are hallmarks of gating modifier toxins, which act by binding to the extracellular end of the S4 voltage sensors of ion channels. The mutation L833C in the S3-S4 linker in domain II reduces affinity for ProTx-II, and mutation of the outermost two gating-charge-carrying arginine residues in the IIS4 voltage sensor to glutamine abolishes voltage-dependent reversal of toxin action and toxin block of gating current. Our results support a voltage-sensor-trapping model for ProTx-II action in which the bound toxin impedes the normal outward gating movement of the IIS4 transmembrane segment, traps the domain II voltage sensor module in its resting state, and thereby inhibits channel activation.

Footnotes

  • This research was supported by Research grant R01-NS15751 and Cooperative Agreement U01-NS058039 from the National Institutes of Health and by a grant from the Binational Research and Development Agency of the United States and Israel.

  • ABBREVIATIONS: ProTx-II, protoxin II; MeSO3, methanesulfonate.

    • Received August 17, 2007.
    • Accepted December 21, 2007.
  • The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 73 (3)
Molecular Pharmacology
Vol. 73, Issue 3
1 Mar 2008
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Research ArticleArticle

Inhibition of Sodium Channel Gating by Trapping the Domain II Voltage Sensor with Protoxin II

Stanislav Sokolov, Richard L. Kraus, Todd Scheuer and William A. Catterall
Molecular Pharmacology March 1, 2008, 73 (3) 1020-1028; DOI: https://doi.org/10.1124/mol.107.041046

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Research ArticleArticle

Inhibition of Sodium Channel Gating by Trapping the Domain II Voltage Sensor with Protoxin II

Stanislav Sokolov, Richard L. Kraus, Todd Scheuer and William A. Catterall
Molecular Pharmacology March 1, 2008, 73 (3) 1020-1028; DOI: https://doi.org/10.1124/mol.107.041046
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