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Molecular Pharmacology

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OtherMINIREVIEW

Fluorescent and Bioluminescent Protein-Fragment Complementation Assays in the Study of G Protein-Coupled Receptor Oligomerization and Signaling

Pierre-Alexandre Vidi and Val J. Watts
Molecular Pharmacology April 2009, 75 (4) 733-739; DOI: https://doi.org/10.1124/mol.108.053819
Pierre-Alexandre Vidi
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Val J. Watts
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Abstract

Most cellular functions, including signaling by G protein-coupled receptors (GPCRs), are mediated by protein-protein interactions, making the identification and localization of protein complexes key to the understanding of cellular processes. In complement to traditional biochemical techniques, noninvasive resonance energy transfer (RET) and protein-fragment complementation assays (PCAs) now allow protein interactions to be detected in the context of living cells. In this review, fluorescent and bioluminescent PCAs are discussed and their application illustrated with studies on GPCR signaling. Newly developed techniques combining PCA and RET assays for the detection of ternary and quaternary protein complexes are also presented.

Footnotes

  • This work was supported by the National Institutes of Health National Institute of Mental Health [Grant MH060397] and by Purdue University.

  • ABBREVIATIONS: FRET, fluorescence (Förster) resonance energy transfer; BRET, bioluminescence resonance energy transfer; BiFC, bimolecular fluorescence complementation; GPCR, G protein-coupled receptor; PCA, protein complementation assay; RET, resonance energy transfer; BiLC, bimolecular luminescence complementation; FP, fluorescent protein; YFP, yellow fluorescent protein; CFP, cyan fluorescent protein; VN, Venus N-terminal fragment; VC, Venus C-terminal fragment; CN, Cerulean N-terminal fragment; CC, Cerulean C-terminal fragment; AR, adrenoceptor; IP, Immunoprecipitation; CRLR, calcitonin receptor-like receptor; ER, endoplasmic reticulum; RAMP, receptor activity-modifying protein; ACII, type II adenylyl cyclase.

    • Received December 6, 2008.
    • Accepted January 13, 2009.
  • The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 75 (4)
Molecular Pharmacology
Vol. 75, Issue 4
1 Apr 2009
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OtherMINIREVIEW

Fluorescent and Bioluminescent Protein-Fragment Complementation Assays in the Study of G Protein-Coupled Receptor Oligomerization and Signaling

Pierre-Alexandre Vidi and Val J. Watts
Molecular Pharmacology April 1, 2009, 75 (4) 733-739; DOI: https://doi.org/10.1124/mol.108.053819

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OtherMINIREVIEW

Fluorescent and Bioluminescent Protein-Fragment Complementation Assays in the Study of G Protein-Coupled Receptor Oligomerization and Signaling

Pierre-Alexandre Vidi and Val J. Watts
Molecular Pharmacology April 1, 2009, 75 (4) 733-739; DOI: https://doi.org/10.1124/mol.108.053819
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  • Article
    • Abstract
    • Principle of Fluorescent and Luminescent PCAs
    • Application of PCA to Study GPCR Oligomerization and Interactions with Modulators
    • PCA as Measure of G Protein Subunit Composition and Trafficking
    • Combining Techniques to Monitor Interactions between Multiple Proteins
    • Conclusions and Outlook
    • Acknowledgments
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