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Molecular Pharmacology

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Research ArticleArticle

An Inhibitor of Protein Arginine Methyltransferases, 7,7′-Carbonylbis(azanediyl)bis(4-hydroxynaphthalene-2-sulfonic acid (AMI-1), Is a Potent Scavenger of NADPH-Oxidase–Derived Superoxide

Feng Chen and David J. R. Fulton
Molecular Pharmacology February 2010, 77 (2) 280-287; DOI: https://doi.org/10.1124/mol.109.061077
Feng Chen
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David J. R. Fulton
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Abstract

The methylation of proteins is an important post-translational mechanism that has been established to influence the activity of nuclear and nucleic acid binding proteins. Much less is known about the importance of protein methylation in the regulation of cytosolic proteins. Increased methylation of proteins is observed in cardiovascular disease and occurs in conjunction with elevated production of reactive oxygen species. However, the nature of the relationship between reactive oxygen species and protein methylation is poorly understood. Therefore, the goal of the current study was to determine whether protein methylation influences the catalytic activity of the NADPH oxidases (Nox), which are a family of enzymes responsible for the generation of superoxide. We found that the selective inhibitor of protein arginine methyltransferases 7,7′-carbonylbis(azanediyl)bis(4-hydroxynaphthalene-2-sulfonic acid (AMI-1) was a potent antagonist of Nox-derived superoxide production. However, structurally and mechanistically dissimilar inhibitors of protein methylation and coexpression of protein arginine methyltransferase 1 did not influence Nox activity. Rather, the effect of AMI-1 was rapidly reversible and could be demonstrated in an assay using chemically synthesized superoxide. We conclude that protein methylation does not regulate the activity of NADPH-oxidases and that AMI-1 is a potent antioxidant with a greater potency than 4,5-dihydroxy-1,3-benzenedisulfonic acid (Tiron) and 4-hydroxy-2,2,6,6-tetramethylpiperydine-1-oxyl (Tempol).

Footnotes

  • Article, publication date, and citation information can be found at http://molpharm.aspetjournals.org.

    doi:10.1124/mol.109.061077

  • ABBREVIATIONS:

    PRMT
    protein arginine methyltransferase
    Nox
    NADPH-oxidase
    AMI-1
    7,7′-carbonylbis(azanediyl)bis(4-hydroxynaphthalene-2-sulfonic acid
    MTA
    5′-deoxy-5′-(methylthio)adenosine
    Sinefungin
    6,9-diamino-1-(6-amino-9H-purin-9-yl)-1,5,6,7,8,9-hexadeoxy-d-glycero-α-l-talo-decafuranuronic acid
    eNOS
    endothelial nitric-oxide synthase
    iNOS
    inducible nitric-oxide synthase
    L-012
    8-amino-5-chloro-7-phenylpyrido(3,4-d)pyridazine-1,4(2H,3H)dione
    MOPS
    3-(N-morpholino)propanesulfonic acid
    PMS
    phenazine methosulfate.

  • Received September 15, 2009.
  • Accepted November 9, 2009.
  • Copyright © 2010 The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 77 (2)
Molecular Pharmacology
Vol. 77, Issue 2
1 Feb 2010
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An Inhibitor of Protein Arginine Methyltransferases, 7,7′-Carbonylbis(azanediyl)bis(4-hydroxynaphthalene-2-sulfonic acid (AMI-1), Is a Potent Scavenger of NADPH-Oxidase–Derived Superoxide
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Research ArticleArticle

An Inhibitor of Protein Arginine Methyltransferases, 7,7′-Carbonylbis(azanediyl)bis(4-hydroxynaphthalene-2-sulfonic acid (AMI-1), Is a Potent Scavenger of NADPH-Oxidase–Derived Superoxide

Feng Chen and David J. R. Fulton
Molecular Pharmacology February 1, 2010, 77 (2) 280-287; DOI: https://doi.org/10.1124/mol.109.061077

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Research ArticleArticle

An Inhibitor of Protein Arginine Methyltransferases, 7,7′-Carbonylbis(azanediyl)bis(4-hydroxynaphthalene-2-sulfonic acid (AMI-1), Is a Potent Scavenger of NADPH-Oxidase–Derived Superoxide

Feng Chen and David J. R. Fulton
Molecular Pharmacology February 1, 2010, 77 (2) 280-287; DOI: https://doi.org/10.1124/mol.109.061077
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