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Molecular Pharmacology

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Research ArticleArticle

Octopamine Receptors from the Barnacle Balanus improvisus Are Activated by the α2-Adrenoceptor Agonist Medetomidine

Ulrika Lind, Magnus Alm Rosenblad, Linda Hasselberg Frank, Susanna Falkbring, Lars Brive, Jonne M. Laurila, Katariina Pohjanoksa, Anne Vuorenpää, Jyrki P. Kukkonen, Lina Gunnarsson, Mika Scheinin, Lena G. E. Mårtensson Lindblad and Anders Blomberg
Molecular Pharmacology August 2010, 78 (2) 237-248; DOI: https://doi.org/10.1124/mol.110.063594
Ulrika Lind
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Magnus Alm Rosenblad
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Linda Hasselberg Frank
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Susanna Falkbring
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Lars Brive
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Jonne M. Laurila
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Katariina Pohjanoksa
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Anne Vuorenpää
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Jyrki P. Kukkonen
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Lina Gunnarsson
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Mika Scheinin
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Lena G. E. Mårtensson Lindblad
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Anders Blomberg
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Abstract

G protein-coupled octopamine receptors of insects and other invertebrates represent counterparts of adrenoceptors in vertebrate animals. The α2-adrenoceptor agonist medetomidine, which is in clinical use as a veterinary sedative agent, was discovered to inhibit the settling process of barnacles, an important step in the ontogeny of this crustacean species. Settling of barnacles onto ship hulls leads to biofouling that has many harmful practical consequences, and medetomidine is currently under development as a novel type of antifouling agent. We now report that medetomidine induces hyperactivity in the barnacle larvae to disrupt the settling process. To identify the molecular targets of medetomidine, we cloned five octopamine receptors from the barnacle Balanus improvisus. We show by phylogenetic analyses that one receptor (BiOctα) belongs to the α-adrenoceptor-like subfamily, and the other four (BiOctβ-R1, BiOctβ-R2, BiOctβ-R3, and BiOctβ-R4) belong to the β-adrenoceptor-like octopamine receptor subfamily. Phylogenetic analyses also indicated that B. improvisus has a different repertoire of β-adrenoceptor-like octopamine receptors than insects. When expressed in CHO cells, the cloned receptors were activated by both octopamine and medetomidine, resulting in increased intracellular cAMP or calcium levels. Tyramine activated the receptors but with much lesser potency than octopamine. A hypothesis for receptor discrimination between tyramine and octopamine was generated from a homology three-dimensional model. The characterization of B. improvisus octopamine receptors is important for a better functional understanding of these receptors in crustaceans as well as for practical applications in development of environmentally sustainable antifouling agents.

Footnotes

  • ↵Embedded Image The online version of this article (available at http://molpharm.aspetjournals.org) contains supplemental material.

  • This work is a subproject within the Marine Paint program financially supported by the Swedish Foundation for Strategic Environmental Research (MISTRA) [Grant 2001-012].

  • Article, publication date, and citation information can be found at http://molpharm.aspetjournals.org.

    doi:10.1124/mol.110.063594.

  • ABBREVIATIONS:

    GPCR
    G protein-coupled receptor
    CHO
    Chinese hamster ovary
    GTPγS
    guanosine 5′-O-(3-thio)triphosphate
    PCR
    polymerase chain reaction
    TM
    transmembrane helix
    RACE
    rapid amplification of cDNA ends
    3D
    three-dimensional
    Oct/Tyr
    octopamine/tyramine.

  • Received January 22, 2010.
  • Accepted May 17, 2010.
  • Copyright © 2010 The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 78 (2)
Molecular Pharmacology
Vol. 78, Issue 2
1 Aug 2010
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Research ArticleArticle

Octopamine Receptors from the Barnacle Balanus improvisus Are Activated by the α2-Adrenoceptor Agonist Medetomidine

Ulrika Lind, Magnus Alm Rosenblad, Linda Hasselberg Frank, Susanna Falkbring, Lars Brive, Jonne M. Laurila, Katariina Pohjanoksa, Anne Vuorenpää, Jyrki P. Kukkonen, Lina Gunnarsson, Mika Scheinin, Lena G. E. Mårtensson Lindblad and Anders Blomberg
Molecular Pharmacology August 1, 2010, 78 (2) 237-248; DOI: https://doi.org/10.1124/mol.110.063594

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Research ArticleArticle

Octopamine Receptors from the Barnacle Balanus improvisus Are Activated by the α2-Adrenoceptor Agonist Medetomidine

Ulrika Lind, Magnus Alm Rosenblad, Linda Hasselberg Frank, Susanna Falkbring, Lars Brive, Jonne M. Laurila, Katariina Pohjanoksa, Anne Vuorenpää, Jyrki P. Kukkonen, Lina Gunnarsson, Mika Scheinin, Lena G. E. Mårtensson Lindblad and Anders Blomberg
Molecular Pharmacology August 1, 2010, 78 (2) 237-248; DOI: https://doi.org/10.1124/mol.110.063594
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