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Research ArticleArticle

Activation of Trimeric P2X2 Receptors by Fewer than Three ATP Molecules

Olga Stelmashenko, Ulyana Lalo, Yue Yang, Laricia Bragg, R. Alan North and Vincent Compan
Molecular Pharmacology October 2012, 82 (4) 760-766; DOI: https://doi.org/10.1124/mol.112.080903
Olga Stelmashenko
Faculty of Medical and Human Sciences (O.S., U.L., L.B., R.A.N.), and Faculty of Life Sciences (Y.Y., R.A.N., V.C.), University of Manchester, Manchester, United Kingdom
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Ulyana Lalo
Faculty of Medical and Human Sciences (O.S., U.L., L.B., R.A.N.), and Faculty of Life Sciences (Y.Y., R.A.N., V.C.), University of Manchester, Manchester, United Kingdom
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Yue Yang
Faculty of Medical and Human Sciences (O.S., U.L., L.B., R.A.N.), and Faculty of Life Sciences (Y.Y., R.A.N., V.C.), University of Manchester, Manchester, United Kingdom
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Laricia Bragg
Faculty of Medical and Human Sciences (O.S., U.L., L.B., R.A.N.), and Faculty of Life Sciences (Y.Y., R.A.N., V.C.), University of Manchester, Manchester, United Kingdom
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R. Alan North
Faculty of Medical and Human Sciences (O.S., U.L., L.B., R.A.N.), and Faculty of Life Sciences (Y.Y., R.A.N., V.C.), University of Manchester, Manchester, United Kingdom
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Vincent Compan
Faculty of Medical and Human Sciences (O.S., U.L., L.B., R.A.N.), and Faculty of Life Sciences (Y.Y., R.A.N., V.C.), University of Manchester, Manchester, United Kingdom
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Abstract

P2X receptors are trimeric membrane proteins. When they bind extracellular ATP, a conformational change occurs that opens a transmembrane ion channel. The ATP-binding pocket is formed in a cleft between two subunits, and a critical amino acid residue for ATP contact is Lys69 (P2X2 numbering). In the present work, we sought to determine whether the binding of fewer than three ATP molecules could open the ion channel. We expressed eight concatenated cDNAs in human embryonic kidney cells, which encoded three serially joined, epitope-tagged, subunits with either Lys or Ala at position 69 (denoted as KKK, KKA, KAK, AKK, KAA, AKA, AAK, and AAA). Western blotting of surface-biotinylated proteins indicated that breakdown of concatemers to individual subunits was minimal. Recording of membrane currents in response to ATP (whole cell and excised outside-out patch) showed that all formed functional channels except AAK, AKA, and AAA. There was no difference in the kinetics of activation and deactivation among KKK, KKA, KAK, and AKK channels, and amplitude of the unitary conductances was in all cases not different from that found after expression of a single wild-type subunit. Currents through KKA and KAK receptors were larger than those observed for AKK receptors. The results indicate that trimeric P2X receptors containing only two intact binding sites can be readily activated by ATP.

Footnotes

  • This work was supported by the Wellcome Trust [Grant 093140/Z/10/Z].

  • Article, publication date, and citation information can be found at http://molpharm.aspetjournals.org.

    http://dx.doi.org/10.1124/mol.112.080903.

  • ABBREVIATIONS:

    HEK
    human embryonic kidney
    sulfo-NHS-LC-biotin
    sulfo-N-hydroxysulfosuccinimide-LC-biotin
    sulfo-NHS-SDA
    N-hydroxysulfosuccinimide diazirine
    NHS-SDA
    N-hydroxysuccinimide diazirine
    KKK
    P2X2 receptor formed from three concatenated wild-type subunits
    AKK
    KAK, and KKA, P2X2 receptors formed from three concatenated subunits in which the first, second, or third such subunits contained K69A substitution
    KAA
    AKA, and AAK, P2X2 receptors formed from three concatenated subunits in which the second and third, first and third, or first and second such subunits contained K69A substitution
    AAA
    P2X2 receptor formed from three concatenated subunits in which all three subunits contained K69A substitution.

  • Received June 29, 2012.
  • Accepted July 24, 2012.
  • Copyright © 2012 The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 82 (4)
Molecular Pharmacology
Vol. 82, Issue 4
1 Oct 2012
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Research ArticleArticle

P2X Receptors Activated by Fewer than Three ATP Molecules

Olga Stelmashenko, Ulyana Lalo, Yue Yang, Laricia Bragg, R. Alan North and Vincent Compan
Molecular Pharmacology October 1, 2012, 82 (4) 760-766; DOI: https://doi.org/10.1124/mol.112.080903

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Research ArticleArticle

P2X Receptors Activated by Fewer than Three ATP Molecules

Olga Stelmashenko, Ulyana Lalo, Yue Yang, Laricia Bragg, R. Alan North and Vincent Compan
Molecular Pharmacology October 1, 2012, 82 (4) 760-766; DOI: https://doi.org/10.1124/mol.112.080903
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