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Research ArticleArticle

Acetylcholine-Binding Protein Engineered to Mimic the α4-α4 Binding Pocket in α4β2 Nicotinic Acetylcholine Receptors Reveals Interface Specific Interactions Important for Binding and Activity

Azadeh Shahsavar, Philip K. Ahring, Jeppe A. Olsen, Christian Krintel, Jette S. Kastrup, Thomas Balle and Michael Gajhede
Molecular Pharmacology October 2015, 88 (4) 697-707; DOI: https://doi.org/10.1124/mol.115.098061
Azadeh Shahsavar
Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark (A.S., J.A.O., C.K., J.S.K., M.G.); Faculty of Pharmacy, University of Sydney, Sydney, New South Wales, Australia (P.K.A., J.A.O., T.B.); Saniona AB, Ballerup, Denmark (P.K.A.); and NeuroSearch A/S, Hellerup, Denmark (J.A.O.)
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Philip K. Ahring
Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark (A.S., J.A.O., C.K., J.S.K., M.G.); Faculty of Pharmacy, University of Sydney, Sydney, New South Wales, Australia (P.K.A., J.A.O., T.B.); Saniona AB, Ballerup, Denmark (P.K.A.); and NeuroSearch A/S, Hellerup, Denmark (J.A.O.)
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Jeppe A. Olsen
Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark (A.S., J.A.O., C.K., J.S.K., M.G.); Faculty of Pharmacy, University of Sydney, Sydney, New South Wales, Australia (P.K.A., J.A.O., T.B.); Saniona AB, Ballerup, Denmark (P.K.A.); and NeuroSearch A/S, Hellerup, Denmark (J.A.O.)
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Christian Krintel
Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark (A.S., J.A.O., C.K., J.S.K., M.G.); Faculty of Pharmacy, University of Sydney, Sydney, New South Wales, Australia (P.K.A., J.A.O., T.B.); Saniona AB, Ballerup, Denmark (P.K.A.); and NeuroSearch A/S, Hellerup, Denmark (J.A.O.)
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Jette S. Kastrup
Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark (A.S., J.A.O., C.K., J.S.K., M.G.); Faculty of Pharmacy, University of Sydney, Sydney, New South Wales, Australia (P.K.A., J.A.O., T.B.); Saniona AB, Ballerup, Denmark (P.K.A.); and NeuroSearch A/S, Hellerup, Denmark (J.A.O.)
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Thomas Balle
Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark (A.S., J.A.O., C.K., J.S.K., M.G.); Faculty of Pharmacy, University of Sydney, Sydney, New South Wales, Australia (P.K.A., J.A.O., T.B.); Saniona AB, Ballerup, Denmark (P.K.A.); and NeuroSearch A/S, Hellerup, Denmark (J.A.O.)
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Michael Gajhede
Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark (A.S., J.A.O., C.K., J.S.K., M.G.); Faculty of Pharmacy, University of Sydney, Sydney, New South Wales, Australia (P.K.A., J.A.O., T.B.); Saniona AB, Ballerup, Denmark (P.K.A.); and NeuroSearch A/S, Hellerup, Denmark (J.A.O.)
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Article Information

vol. 88 no. 4 697-707
DOI 
https://doi.org/10.1124/mol.115.098061
PubMed 
26180047

Published By 
American Society for Pharmacology and Experimental Therapeutics
Print ISSN 
0026-895X
Online ISSN 
1521-0111
History 
  • Received January 23, 2015
  • Accepted July 15, 2015
  • Published online August 21, 2015.

Article Versions

  • Earlier version (July 15, 2015 - 06:15).
  • You are viewing the most recent version of this article.
Copyright & Usage 
Copyright © 2015 by The American Society for Pharmacology and Experimental Therapeutics

Author Information

  1. Azadeh Shahsavar,
  2. Philip K. Ahring,
  3. Jeppe A. Olsen,
  4. Christian Krintel,
  5. Jette S. Kastrup,
  6. Thomas Balle, and
  7. Michael Gajhede
  1. Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark (A.S., J.A.O., C.K., J.S.K., M.G.); Faculty of Pharmacy, University of Sydney, Sydney, New South Wales, Australia (P.K.A., J.A.O., T.B.); Saniona AB, Ballerup, Denmark (P.K.A.); and NeuroSearch A/S, Hellerup, Denmark (J.A.O.)
  1. Address correspondence to:
    Dr. Michael Gajhede, Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, Denmark. E-mail: mig{at}sund.ku.dk or Dr. Thomas Balle, Faculty of Pharmacy, A15, University of Sydney, NSW 2006, Australia. E-mail: thomas.balle{at}sydney.edu.au
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Molecular Pharmacology: 88 (4)
Molecular Pharmacology
Vol. 88, Issue 4
1 Oct 2015
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Research ArticleArticle

Engineered Ls-AChBP with an α4-α4 Binding Pocket

Azadeh Shahsavar, Philip K. Ahring, Jeppe A. Olsen, Christian Krintel, Jette S. Kastrup, Thomas Balle and Michael Gajhede
Molecular Pharmacology October 1, 2015, 88 (4) 697-707; DOI: https://doi.org/10.1124/mol.115.098061

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Research ArticleArticle

Engineered Ls-AChBP with an α4-α4 Binding Pocket

Azadeh Shahsavar, Philip K. Ahring, Jeppe A. Olsen, Christian Krintel, Jette S. Kastrup, Thomas Balle and Michael Gajhede
Molecular Pharmacology October 1, 2015, 88 (4) 697-707; DOI: https://doi.org/10.1124/mol.115.098061
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