Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

Recovery of Rat Adrenal Amine Stores after Insulin Administration

THEODORE A. SLOTKIN and NORMAN KIRSHNER
Molecular Pharmacology January 1973, 9 (1) 105-116;
THEODORE A. SLOTKIN
Department of Physiology and Pharmacology and Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
NORMAN KIRSHNER
Department of Physiology and Pharmacology and Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Acute adrenal medullary discharge of catecholamines was produced in response to the hypoglycemia caused by a large dose of insulin (5 IU/kg, intravenously). Rats were killed 4, 24, 48, 72, or 96 hr after drug administration. Intact adrenal storage vesicles were separated from empty vesicles by differential and density gradient centrifugation, and all fractions were analyzed for catecholamines, dopamine β-hydroxylase (a marker for vesicle membranes), and ATP. Four hours after insulin, vesicular catecholamines and ATP fell to 25% of control levels, while vesicular dopamine β-hydroxylase fell to 40%; the enzyme activity increased in the broken vesicle membrane fraction. The ability of isolated storage vesicles to incorporate [14C]epinephrine fell to about 25% of controls, while incorporation of [3H]metaraminol fell only to 65%. Twenty-four hours after insulin administration vesicular ATP, dopamine β-hydroxylase, and [14C]epinephrine incorporation returned to 50-60% of controls, while [3H]metaraminol incorporation returned to normal; vesicular catecholamine content remained at about 25%. Catecholamine recovery consistently lagged behind all other parameters; by 96 hr vesicular catecholamines, ATP, and [14C]epinephrine incorporation approached control levels, while vesicular dopamine β-hydroxylase exceeded control values. Isopycnic sucrose density gradient centrifugation of vesicles from control rats, and from rats given insulin 24 hr previously, indicated that the newly synthesized storage vesicles were able to incorporate both isotopically labeled epinephrine and metaraminol, but that the new vesicles had a lower equilibrium density than the original population of vesicles. They also had a lower catecholamine content and a lower specificity for epinephrine relative to metaraminol than in the controls. The efflux of endogenous or newly incorporated epinephrine from the new vesicles was nearly identical with that of the controls. These data suggest that the sequence of recovery of adrenal amine stores after sympathetic discharge proceeds by the following process: (a) resynthesis of vesicles (reappearance of vesicular dopamine β-hydroxylase) with restoration of ability to incorporate metaraminol; (b) restoration of storage mechanism (ATP) and epinephrine incorporation; (c) restoration of catecholamines; (d) oversynthesis of new vesicles (as determined by dopamine β-hydroxylase measurements).

ACKNOWLEDGMENT The authors wish to thank Mrs. Nannie Jordan for her technical assistance.

  • Copyright ©, 1973, by Academic Press, Inc.

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology
Vol. 9, Issue 1
1 Jan 1973
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Editorial Board (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Recovery of Rat Adrenal Amine Stores after Insulin Administration
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

Recovery of Rat Adrenal Amine Stores after Insulin Administration

THEODORE A. SLOTKIN and NORMAN KIRSHNER
Molecular Pharmacology January 1, 1973, 9 (1) 105-116;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

Recovery of Rat Adrenal Amine Stores after Insulin Administration

THEODORE A. SLOTKIN and NORMAN KIRSHNER
Molecular Pharmacology January 1, 1973, 9 (1) 105-116;
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Therapeutic Effects of FGF23 Antagonists in Hyp Mice
  • TRPV3 and TRPV4 Channels Coassemble into Heterotetramers
  • Secretin Amino-Terminal Structure-Activity Relationships and Complementary Mutagenesis at the Site of Docking to the Secretin Receptor
Show more Article

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2022 by the American Society for Pharmacology and Experimental Therapeutics