Abstract
Antibody-producing lymphoid cells incubated in vitro in the presence of [3H]lysergic acid diethylamide secrete labeled protein. Incorporation of [3H]lysergic acid diethylamide (or a derivative) de novo was indicated by precipitation of labeled peptides with trichloracetic acid and by the retention of label after dialysis against denaturing agents and high concentrations of a dissociating ligand. Incorporation of [3H]lysergic acid diethylamide by immune lymphoid cells was inhibited by puromycin and enhanced by homologous antigen.
ACKNOWLEDGMENT We thank Dennis E. Lopatin for performing the equilibrium dialysis experiments in microdialysis chambers.
- Copyright ©, 1973, by Academic Press, Inc.
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