Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

Reversibility of the Interaction of Strophanthidin Bromoacetate with the Cardiotonic Steroid Binding Site of Sodium- and Potassium-Dependent Adenosine Triphosphatase

THOMAS TOBIN, TAI AKERA, DAVID KU and MATTHIAS C. LU
Molecular Pharmacology September 1973, 9 (5) 676-685;
THOMAS TOBIN
Department of Pharmacology, Michigan State University, East Lansing, Michigan 48823
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
TAI AKERA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DAVID KU
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
MATTHIAS C. LU
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

The interaction of strophanthidin bromoacetate (SBA) with rat brain (Na+ + K+)-ATPase ATP phosphohydrolase, EC 3.6.1.3) was investigated. SBA displaced [3H]ouabain from the Na+-, Mg++-, and ATP-dependent binding site on the enzyme. The apparent affinity of SBA for the cardiotonic steroid binding site of the enzyme was at least one-third of that of ouabain for this site. When SBA was allowed to bind to the cardiotonic steroid binding site in the presence of Na+, Mg++, and ATP or of Mg++ and Pi, [3H]ouabain added at various intervals after SBA equilibrated with all the cardiotonic steroid binding sites. If SBA was allowed to bind to the enzyme in the presence of Mg++ and Pi or of Na+, Mg++, and UTP, its subsequent phosphorylation form [γ-32P]ATP was reduced by 60-80%. Exposure of this inhibited enzyme to high concentrations of EDTA resulted in recovery of phosphorylation capacity in less than 5 min at 37°. Since SBA did not irreversibly occupy the cardiotonic steroid binding sites or give rise to irreversible inhibition of the enzyme, SBA does not appear to be an effective affinity label for Na+ + K+)-ATPase. Because a challenge to the concept that (Na+ + K+)-ATPase may be the cardiotonic receptor for cardiotonic steroids [B. F. Roth-Schechter, G. T. Okita, R. E. Thomas, and F. F. Richardson, J. Pharmacol. Exp. Ther. 171, 13-19 (1970)] is based on the supposed irreversibility of the SBA-(Na+ + K+)-ATPase interaction, our results leave such an interpretation open to question.

ACKNOWLEDGMENTS The authors would like to thank Dr. T. M. Brody for helpful suggestions and for reviewing the manuscript, and Mrs. Annie Han for excellent technical assistance.

  • Copyright ©, 1973, by Academic Press, Inc.

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology
Vol. 9, Issue 5
1 Sep 1973
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Editorial Board (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Reversibility of the Interaction of Strophanthidin Bromoacetate with the Cardiotonic Steroid Binding Site of Sodium- and Potassium-Dependent Adenosine Triphosphatase
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

Reversibility of the Interaction of Strophanthidin Bromoacetate with the Cardiotonic Steroid Binding Site of Sodium- and Potassium-Dependent Adenosine Triphosphatase

THOMAS TOBIN, TAI AKERA, DAVID KU and MATTHIAS C. LU
Molecular Pharmacology September 1, 1973, 9 (5) 676-685;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

Reversibility of the Interaction of Strophanthidin Bromoacetate with the Cardiotonic Steroid Binding Site of Sodium- and Potassium-Dependent Adenosine Triphosphatase

THOMAS TOBIN, TAI AKERA, DAVID KU and MATTHIAS C. LU
Molecular Pharmacology September 1, 1973, 9 (5) 676-685;
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • The binding site for KCI807 in the androgen receptor
  • Fatty acid amide hydrolase in cisplatin nephrotoxicity
  • eCB Signaling System in hiPSC-Derived Neuronal Cultures
Show more Article

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics