Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

Studies on the Membrane-Perturbational Effects of Drugs and Divalent Cations Utilizing Trinitrobenzenesulfonic Acid

DAVID V. GODIN and THERESE WAN NG
Molecular Pharmacology November 1973, 9 (6) 802-819;
DAVID V. GODIN
Department of Pharmacology, University of British Columbia, Vancouver 8, British Columbia, Caonada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
THERESE WAN NG
Department of Pharmacology, University of British Columbia, Vancouver 8, British Columbia, Caonada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

This study represents an inquiry into the molecular mechanisms whereby the interaction of divalent cations and certain cationic drugs with the erythrocyte membrane produces a marked increase in the modification of membrane amino groups by trinitrobenzenesulfonate (TNBS). A mechanism based on simple neutralization of membrane anionic sites by activator cations was untenable because the incorporation of picryl chloride, an uncharged analogue of TNBS, was also markedly increased in the presence of magnesium or chlorpromazine. Rather, it is suggested that the interaction of activators with membrane components induces configurational changes which alter the accessibility and/or reactivity of membrane protein and phospholipid amino groups. These configurational changes, which were found to require the integrity of both polar and nonpolar regions of phospholipids, may involve alterations in membrane hydration following charge neutralization. The specific membrane perturbations induced by individual activators have been analyzed in terms of effects on the activation energy for TNBS incorporation and also in terms of TNBS labeling patterns of membrane protein and lipid components characteristic of the particular cationic activator.

ACKNOWLEDGMENT We are most grateful to Dr. J. G. Foulks for his interest in this work and for his many helpful suggestions during the preparation of the manuscript.

  • Copyright ©, 1973, by Academic Press, Inc.

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology
Vol. 9, Issue 6
1 Nov 1973
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Studies on the Membrane-Perturbational Effects of Drugs and Divalent Cations Utilizing Trinitrobenzenesulfonic Acid
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

Studies on the Membrane-Perturbational Effects of Drugs and Divalent Cations Utilizing Trinitrobenzenesulfonic Acid

DAVID V. GODIN and THERESE WAN NG
Molecular Pharmacology November 1, 1973, 9 (6) 802-819;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

Studies on the Membrane-Perturbational Effects of Drugs and Divalent Cations Utilizing Trinitrobenzenesulfonic Acid

DAVID V. GODIN and THERESE WAN NG
Molecular Pharmacology November 1, 1973, 9 (6) 802-819;
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • EIPA, HMA and SMN2 gene regulation
  • Clc-2 has minor role in intestinal Cl- secretion
  • Resveratrol acts as an NR4A1 antagonist in lung cancer.
Show more Article

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2022 by the American Society for Pharmacology and Experimental Therapeutics