Abstract
The addition of increasing concentrations of a detergent (Triton X-100)-solubilized cytochrome b5 from liver microsomes to liver microsomes of untreated or phenobarbital-treated animals produced correspondingly greater inhibition of aminopyrine dealkylation. Even addition of NADH to the NADPH-supported reaction did not prevent this inhibition, although the added cytochrome b5 functioned as an endogenous hemoprotein; i.e., it was reduced by NADH and stimulated the reduction of exogenous cytochrome c. These observations suggest that cytochrome b5 does not function in hepatic microsomal mixedfunction oxidase reactions.
- Copyright ©, 1973, by Academic Press, Inc.
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