| μM | | | |
Wild typec | 0.10 (0.08–0.12) | — | | This work |
V474S | 0.88 (0.67–1.2) | 8.8 | On D1-D2 linker, entrance to ATP site, CB-5083 contacting | This work |
I479S | 1.2 (1.1–1.3) | 11.8 | On D1-D2 linker, at ATP site, base and CB-5083 contacting | This work |
L526S | 1.4 (1.3–1.5) | 13.9 | At ATP site, base and CB-5083 contacting | This work |
I656S | 0.15 (0.13–0.19) | 1.5 | At ATP site, base and CB-5083 contacting | This work |
D1-D2d | 0.012 (0.015–0.02) | 0.17 | | This work |
Wild typec | 0.011 | | | Anderson et al. (2015) |
N660K | 0.31 | 28.5 | At ATP site, base and CB-5083 contacting | Anderson et al. (2015) |
A659T | — | | At ATP site, base and CB-5083 contacting | Anderson et al. (2015) |
T688A | 0.64 | 58 | At ATP site, ribose and CB-5083 contacting | Anderson et al. (2015) |
V474A | 0.10 | 9.3 | On D1-D2 linker, entrance to ATP site, CB-5083 contacting | Anderson et al. (2015) |
Q473P | 0.041 | 3.7 | On D1-D2 linker, entrance to ATP site | Anderson et al. (2015) |
P472L | — | | On D1-D2 linker, entrance to ATP site | Anderson et al. (2015) |
Wild typec | 0.013 | | | Bastola et al. (2017) |
E470K | 0.044 | 3.4 | On D1-D2 linker, entrance to ATP site, higher ATPase activity | Bastola et al. (2017) |
E470D | 0.049 | 3.8 | On D1-D2 linker, entrance to ATP site, higher ATPase activity | Bastola et al. (2017) |