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- Supplemental Figures -
Supplementary Figure 1 - Expression of RORα target (G6PC, CYP7B1, and SULT2A1) and non-target gene (CAR) in human primary hepatocytes before and after RORα knockdown by siRNAs. The cells were treated with control or RORα siRNAs for 72 hrs. and total RNAs were extracted using Trizol® reagent. cDNAs were prepared by using reverse transcriptase before mRNA estimation using probes RT-PCR.
Supplementary Figure 2 - (A) CAR enhanced RORα binding onto the OARE motif. ChIP PCR and quantitative ChIP PCR assay was performed in Huh7 cells transiently expressing CAR, RORα or both using anti-RORα antibody. While RORα could be detected on the OARE sequence, cotransfection of CAR and RORα into the cells enhanced the RORα binding at this motif. The left panel shows ChIP-PCR data while the right panel quantitative ChIP PCR results depicts relative chromatin enrichment on the OARE sequence. B. ChIP assay depicting the enhancement of HNF 4α binding at the OARE sequence in Huh7 cells transiently expressing RORα. Huh7 was transfected with CAR or RORa expression vectors. Endogenously expressed HNF4a binding onto the OARE motif was monitored by ChIP PCR.
- Supplemental Figures -