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Research ArticleSpecial Section on Phosphoproteomic Analysis of G Protein-Coupled Pathways - Axelrod Symposium—Minireview

Proteomic Approaches to Investigate Regulated Trafficking and Signaling of G Protein–Coupled Receptors

Mark von Zastrow
Molecular Pharmacology May 2021, 99 (5) 392-398; DOI: https://doi.org/10.1124/molpharm.120.000178
Mark von Zastrow
Departments of Cellular and Molecular Pharmacology, and Psychiatry and Behavioral Science, San Francisco School of Medicine, and Quantitative Biology Institute, University of California, San Francisco, California
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Article Information

vol. 99 no. 5 392-398
DOI 
https://doi.org/10.1124/molpharm.120.000178
PubMed 
33361190

Published By 
American Society for Pharmacology and Experimental Therapeutics
Print ISSN 
0026-895X
Online ISSN 
1521-0111
History 
  • Received October 6, 2020
  • Accepted November 9, 2020
  • Published online April 15, 2021.

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  • Earlier version (December 22, 2020 - 11:19).
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Copyright © 2021 by The American Society for Pharmacology and Experimental Therapeutics

Author Information

  1. Mark von Zastrow
  1. Departments of Cellular and Molecular Pharmacology, and Psychiatry and Behavioral Science, San Francisco School of Medicine, and Quantitative Biology Institute, University of California, San Francisco, California
  1. Address correspondence to:
    Dr. Mark von Zastrow, University of California, UCSF Mission Bay, Genentech Hall Rm. N212E, 600 16th St., San Francisco, CA 94143. E-mail: mark{at}vzlab.org
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Molecular Pharmacology: 99 (5)
Molecular Pharmacology
Vol. 99, Issue 5
1 May 2021
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Research ArticleSpecial Section on Phosphoproteomic Analysis of G Protein-Coupled Pathways - Axelrod Symposium—Minireview

Proteomic Analysis of GPCR Cell Biology

Mark von Zastrow
Molecular Pharmacology May 1, 2021, 99 (5) 392-398; DOI: https://doi.org/10.1124/molpharm.120.000178

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Research ArticleSpecial Section on Phosphoproteomic Analysis of G Protein-Coupled Pathways - Axelrod Symposium—Minireview

Proteomic Analysis of GPCR Cell Biology

Mark von Zastrow
Molecular Pharmacology May 1, 2021, 99 (5) 392-398; DOI: https://doi.org/10.1124/molpharm.120.000178
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  • Article
    • Abstract
    • Introduction
    • GPCR Endocytosis and Signaling Are Intricately Interrelated
    • Use of Quantitative Mass Spectrometry to Elaborate Agonist-Selective Encoding of GPCR Endocytosis by Phosphorylation
    • Use of Affinity Purification Mass Spectrometry to Delineate a Mechanism Mediating Sequence-Directed GPCR Sorting
    • Use of APEX-Mediated Proximity Labeling and Quantitative Mass Spectrometry to Spatiotemporally “Map” Cytoplasmic Protein Interactions with GPCRs
    • Use of Phosphoproteomic Profiling to Investigate Endosomal Signaling by GPCRs
    • Conclusion and Future Directions
    • Acknowledgments
    • Authorship Contributions
    • Footnotes
    • Abbreviations
    • References
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