Article Figures & Data
Figures
Additional Files
Data Supplement
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Supplemental Figure 1 - A. Representative phase contrast images from InCucyte of CUTO22, CUTO32, CUTO42 and LC-2/Ad cells under untreated conditions show different cellular morphologies.
Supplemental Figure 2 - RET inhibitors rapidly decrease RET activation and is sustained for up to 24 hours.
Supplemental Figure 3 - CUTO32 cells treated with indicated concentrations of ponatinib, RXDX-105 or BLU-667 for 2 hours.
Supplemental Figure 4 - . CUTO32 cells are sensitive to PLK1 and Aurora kinase inhibitors in drug screen.
Supplemental Figure 5 - Normalized expression in counts per million (CPM) of selected genes from RNA sequencing.
Supplemental Figure 6 - Co-inhibition of MET does not sensitize RET+ cells to RET inhibitors.
Supplemental Figure 7 - Average mouse weight measurements for xenograft experiments.
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Data Supplement
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Supplemental Methods
Supplemental Table 1 - Sequences for knockdown, knock-out, protein inhibitor experiments.
Supplemental Table 2 - Characteristics of cell lines used in hUNG2 inhibition experiments and FdU IC50
valuesaSupplemental Table 3 - Substrate sequences for hUNG activity assay.
Supplemental Table 4 - Retention time and fragmentation products monitored by LC-MS.
Supplemental Table 5 - IC50 values for FU in colorectal cancer cell linesa
Supplement Table 6 - Raltitrexed IC50 values and Area under curve analysis for Colorectal Cancer Cell Linesa.Supplemental Figure 1 - Validation of HCT116 isogenic TP53 knock-out.
Supplemental Figure 2 - Measurement of hUNG Activity for non-colorectal cell lines.
Supplemental Figure 3 - Measurement of Inducible hUNG inhibition after 3 months of cultures.
Supplemental Figure 4 - Inhibition of hUNG activity increases potency of FdU for a subset of noncolorectal cancer cell lines.
Supplemental Figure 5 - Average transcript levels of DNA damage repair and uracil metabolism genes in hUNG-responsive and non-responsive cancer cell lines.
Supplemental Figure 6 - Evaluation of apoptosis and double stranded breaks in hUNG responsive and nonresponsive colon cancer cell lines in response to 100 nM concentration of FdU.
Supplemental Figure 7 - Validation of Apoptosis Assay using TRAIL agonist.
Supplemental Figure 8 - Cell proliferation assay for FdU toxicity in representative R and NR cell lines.
Supplemental Figure 9 - Quantitative measurements of hUNG activity across FdU responsive and non-responsive cell lines.
Supplemental Figure 10 - Sanger electropherogram tracings of thymine-DNA glycosylase (TDG) CRISPR knock out clones in the DLD1 background.
Supplemental Figure 11 - Evaluating the impact of ion channel blockade or NOTCH1 inhibition to exacerbate FdU cytotoxicity in two R lines.
Supplemental References
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Data Supplement
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Supplemental Figure 1 - Structure of Ang II and its chemicaly modified analogues.
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