Supplemental Data

Files in this Data Supplement:

• Supplemental Data -

  Supplemental Materials and Methods

  Supplemental Figure 1 - Chemistry of salicylic acid immobilization

  Supplemental Figure 2 - Species- and cell type-independent interaction of FECH with salicylic acid

  Supplemental Figure 3 - Gel filtration and crystallization

  Supplemental Figure 4 - Analysis of binding parameters between R115L FECH and salicylic acid by ITC

  Supplemental Figure 5 - Knockdown of zfech or salicylic acid treatment does not cause gross morphological abnormalities in zebrafish embryos

  Supplemental Figure 6 - Multiple sequence alignment of FECH protein sequence from human (hFECH), Escherichia coli (eFECH) and zebrafish (zFECH)

  Supplemental Figure 7 - Complete structure of the FECH-salicylic acid complex

  Supplemental Table 1 - Data collection and refinement statistics

  Supplemental Table 2 - Selected thermodynamic parameters for the FECH-salicylic acid interaction