Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

Multiple birds with one stone: microRNA hsa-miR-1301-3p regulates human ADH6, ALDH5A1 and ALDH8A1 in the ethanol-acetaldehyde-acetate metabolic pathway

Xubing Wang, Yanjie Zhao, Jiao Luo, Lin Xu, Xinmei Li, Yuan Jin, Chuanhai Li, Meiyao Feng, Ying Wang, Jing Chen, Yufei Hou, Qianwen Zhao, Jinquan Zhao, Baitang Ning, Yuxin Zheng and Dianke Yu
Molecular Pharmacology June 4, 2020, mol.120.119693; DOI: https://doi.org/10.1124/mol.120.119693
Xubing Wang
1 School of Public Health, Qingdao University;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yanjie Zhao
1 School of Public Health, Qingdao University;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jiao Luo
1 School of Public Health, Qingdao University;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lin Xu
1 School of Public Health, Qingdao University;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xinmei Li
1 School of Public Health, Qingdao University;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yuan Jin
1 School of Public Health, Qingdao University;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chuanhai Li
1 School of Public Health, Qingdao University;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Meiyao Feng
1 School of Public Health, Qingdao University;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ying Wang
1 School of Public Health, Qingdao University;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jing Chen
1 School of Public Health, Qingdao University;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yufei Hou
1 School of Public Health, Qingdao University;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Qianwen Zhao
1 School of Public Health, Qingdao University;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jinquan Zhao
1 School of Public Health, Qingdao University;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Baitang Ning
2 National Center for Toxicological Research, US Food and Drug Administration.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yuxin Zheng
1 School of Public Health, Qingdao University;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dianke Yu
1 School of Public Health, Qingdao University;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF + SI
  • PDF
Loading

Abstract

Alcohol dehydrogenases (ADHs) and aldehyde dehydrogenases (ALDHs) are vital enzymes involved in the metabolism of a variety of alcohols. Differences in the expression and enzymatic activity of human ADHs and ALDHs correlate with individual variability in metabolizing alcohols and drugs and in the susceptibility to alcoholic liver disease (ALD). MicroRNAs (miRNAs) function as epigenetic modulators to regulate the expression of drug metabolizing enzymes (DMEs). To characterize miRNAs that target ADHs and ALDHs in human liver cells, we carried out a systematic bioinformatics analysis to analyze free energies of the interaction between miRNAs and their cognate sequences in ADH and ALDH transcripts, and then calculated expression correlations between miRNAs and their targeting ADH and ALDH genes using a public database. Candidate miRNAs were selected to evaluate bioinformatic predictions using a series of biochemical assays. Our results showed that 11 miRNAs have the potential to modulate the expression of 2 ADH and 7 ALDH genes in the human liver. We found that hsa-miR-1301-3p suppressed the expression of ADH6, ALDH5A1, and ALDH8A1 in liver cells and blocked their induction by ethanol. In summary, our results revealed that hsa-miR-1301-3p plays an important role in ethanol metabolism by regulating ADH and ALDH gene expression.

SIGNIFICANCE STATEMENT We showed that 11 miRNAs might play regulatory roles in the expression of 2 ADH and 7 ALDH genes in human liver via systematic bioinformatics analysis. Experimental evidences proved that hsa-miR-1301-3p suppressed the expression of ADH6, ALDH5A1, and ALDH8A1 in liver cells and blocked their induction by ethanol.

  • Gene regulation
  • Hepatoxicity
  • MicroRNA
  • The American Society for Pharmacology and Experimental Therapeutics
Next
Back to top

In this issue

Molecular Pharmacology: 102 (2)
Molecular Pharmacology
Vol. 102, Issue 2
1 Aug 2022
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Multiple birds with one stone: microRNA hsa-miR-1301-3p regulates human ADH6, ALDH5A1 and ALDH8A1 in the ethanol-acetaldehyde-acetate metabolic pathway
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

Multiple birds with one stone: microRNA hsa-miR-1301-3p regulates human ADH6, ALDH5A1 and ALDH8A1 in the ethanol-acetaldehyde-acetate metabolic pathway

Xubing Wang, Yanjie Zhao, Jiao Luo, Lin Xu, Xinmei Li, Yuan Jin, Chuanhai Li, Meiyao Feng, Ying Wang, Jing Chen, Yufei Hou, Qianwen Zhao, Jinquan Zhao, Baitang Ning, Yuxin Zheng and Dianke Yu
Molecular Pharmacology June 4, 2020, mol.120.119693; DOI: https://doi.org/10.1124/mol.120.119693

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

Multiple birds with one stone: microRNA hsa-miR-1301-3p regulates human ADH6, ALDH5A1 and ALDH8A1 in the ethanol-acetaldehyde-acetate metabolic pathway

Xubing Wang, Yanjie Zhao, Jiao Luo, Lin Xu, Xinmei Li, Yuan Jin, Chuanhai Li, Meiyao Feng, Ying Wang, Jing Chen, Yufei Hou, Qianwen Zhao, Jinquan Zhao, Baitang Ning, Yuxin Zheng and Dianke Yu
Molecular Pharmacology June 4, 2020, mol.120.119693; DOI: https://doi.org/10.1124/mol.120.119693
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF + SI
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • α-Conotoxin Binding Site on the GABAB Receptor
  • Upacicalcet binds to the amino acid binding site of CaSR
  • Characterization of GRD and LCCH3 from Human Louse
Show more Article

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2022 by the American Society for Pharmacology and Experimental Therapeutics