Article Figures & Data
Additional Files
Data Supplement
- Supplemental Data -
Supplementary Figure 1 - Effect of mutations on CYP2B6V5 basal expression and activity.
Supplementary Figure 2 - Down-regulation of CYP2B6V5 and its mutant proteins in HuH7 cells.
Supplementary Figure 3 - Time dependent down-regulation of CYP2B6 and Y203A, Y380A mutants by DPTA.
Supplementary Figure 4 - DPTA-stimulated formation of HMM species is inhibited on CYP2B6V5-Y380A and -317A mutant enzymes.
Supplementary Figure 5 - Equilibrium dynamics of CYP2B6v5 systems.
Supplementary Figure 6 - Secondary structure of CYP2B6v5 and 3-nitrotyrosine systems during the last nanosecond of each simulation.
Supplementary Figure 7 - Secondary structure of CYP2B6v5 and alanine mutants during the last nanosecond of each simulation.
Supplementary Figure 8 - Lack of effect of decomposed CORM2 on HMM formation.
Supplementary Figure 9 - Effect of carbon monoxide releasing molecule 2 (CORM2) on HMM species formation in WT-2B6V5, Y203A and Y380A mutants in HuH7 cell lines.
Supplementary Figure 10 - No rapid reversal of 2B6 inhibition after DPTA washout.
Supplementary Figure 11 - Slow recovery of 2B6 activity.
Supplementary Figure 12 - In vitro tyrosine nitration of CYP2B6V5 and its mutants.
- Supplemental Data -
Supplementary Data 1 - 3-Nitrotyrosine coordinate file (.pdb)
- Supplemental Data -
Supplementary Data 2. 3-Nitrotyrosine topology file (.itp )
- Supplemental Data -
Supplementary Data 3. Molecular model of CYP2B6 TYR190NIY
- Supplemental Data -
Supplementary Data 4- Molecular model of CYP2B6 TYR235NIY
- Supplemental Data -
Supplementary Data 5 - Molecular model of CYP2B6 TYR317NIY
- Supplemental Data -
Supplementary Data 6 - Molecular model of CYP2B6 TYR380NIY
- Supplemental Data -
Supplementary Data 7 - Molecular model of CYP2B6 TYR190ALA
- Supplemental Data -
Supplementary Data 8 - Molecular model of CYP2B6 TYR235ALA
- Supplemental Data -
Supplementary Data 9 - Molecular model of CYP2B6 TYR317ALA
- Supplemental Data -
Supplementary Data 10 - Molecular model of CYP2B6 TYR380ALA
- Supplemental Data -
Supplementary Data 11 - Molecular model of CYP2B6 TYR235NIY Y226H/K262R
- Supplemental Data -
Supplementary Data 12 - Statistical analyses
- Supplemental Data -