Data Supplement

• Supplemental Data -

  Supplemental Fig. S1. Sanger sequencing of the coding regions of the NT5C2 gene (A) and PRPS1 gene (B) in parental cells and the NT5C2-R39Q or PRPS1-S103N mutated subline.
  

  Supplemental Fig. S2. Off-target edit analysis.
  

  Supplemental Fig. S3. Sanger sequencing of the genomic PCR products after TA cloning in NT5C2-mutated sublines.
  

  Supplemental Fig. S4. Inframe indels and exon skipping due to non-homologous end-joining at the target site in
  PRPS1-mutated sublines.
  

  Supplemental Fig. S5. Reduced apoptosis after 7-day exposure to 200 nM of 6-MP in NT5C2-mutated sublines N#2-3 and PRPS1-mutated sublines P#3-5.
  

  Supplemental Table S1. Primer for PCR and RT-PCR analyses.
  

  Supplemental Table S2. Incidences of NT5C2-R39Q and PRPS1-S103N mutations after exposure to 6-MP.
  

  Supplemental Table S3. Cell viability (%) under combined treatment of MPA with 6-MP.