Abstract
Addition of the synthetic chemotactic peptide, fMet-Leu-Phe, to rabbit neutrophils elevates the basal level of cyclic 3',5'-AMP. Stimulation of the cyclic AMP level reaches a maximum at 1 min (60% increase over basal value at 5 nM fMet-Leu-Phe) and subsequently the cyclic AMP level returns to the basal value within 5 min. In the absence of extracellular Ca2+, the cyclic AMP level increases minimally (<20%) upon exposure to fMet-Leu-Phe. The effect of fMet-Leu-Phe is detectable at 0.5 nM and increases at higher concentrations. Inhibition of the cyclic AMP-dependent phosphodiesterase activity by pretreatment with 1-methyl-3-isobutylxanthine enhances both the basal and stimulated cyclic AMP levels.
ACKNOWLEDGMENTS We thank Dr. P. H. Naccache for many timely discussions and Dr. M. C. Wacholtz for helpful information in the course of this work. We also thank Dr. R. J. Freer for supplying the chemotactic peptide.
- Copyright © 1979 by Academic Press, Inc.
MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years.Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page.
|