Abstract
One member of the OATP family of transporters, rat Oatp1, functions as an anion exchanger that is driven in part by the glutathione (GSH) electrochemical gradient, indicating that other OATP-related transporters may also be energized by this mechanism. The present study examined whether rat Oatp2 is also an anion exchanger, and, if so, whether it is energized by the GSH electrochemical gradient. As with Oatp1, uptake of 10 μM [3H]taurocholate in Oatp2-expressing Xenopus laevis oocytes wastrans-stimulated by intracellular 0.2 mM unlabeled taurocholate, indicating bidirectional transport. Interestingly, [3H]taurocholate uptake in Oatp2-expressing oocytes was also trans-stimulated when oocytes were preloaded with GSH, S-methylglutathione,S-sulfobromophthalein-glutathione,S-dinitrophenyl glutathione, or ophthalmic acid (a GSH analog) but not by glutarate or N-acetylcysteine, suggesting that GSH derivatives and conjugates may function as intracellular substrates for Oatp2. Support for this hypothesis was provided by the demonstration of enhanced [3H]GSH and [3H]S-(2,4-dinitrophenyl)-glutathione efflux in Oatp2-expressing oocytes. However, in contrast to Oatp1, extracellular GSH failed to cis-inhibit uptake of [3H]taurocholate or [3H]digoxin in Oatp2-expressing oocytes, indicating that the stimulatory effect of high intracellular GSH concentrations is not due to a coupled exchange mechanism. Taken together, the results indicate that Oatp2 mediates bidirectional transport of organic anions by a GSH-sensitive facilitative diffusion mechanism and suggest that this transporter may play a role in cellular export of specific organic molecules.
Footnotes
- Received February 4, 2000.
- Accepted May 8, 2000.
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Send reprint requests to: Ned Ballatori, Ph.D., Department of Environmental Medicine, Box EHSC, University of Rochester School of Medicine, 575 Elmwood Ave., Rochester, NY 14642. E-mail:Ned_Ballatori{at}urmc.rochester.edu
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This work was supported in part by National Institutes of Health Grants DK48823 and ES06484, National Institute of Environmental Health Sciences Center Grant ES01247, and Swiss National Science Foundation Grant 31-45536.95.
- The American Society for Pharmacology and Experimental Therapeutics
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