Abstract
Various methods for estimating the stoichiometric relationship between drug oxidation and NADPH oxidation in hepatic microsomes are discussed. Of these, two methods give values approaching 1:1 for a number of type I substrates. One method relates the metabolism to the substrate-dependent NADPH oxidation corrected for CO-sensitive, endogenous NADPH oxidation. The correction was obtained by measuring the rate of NADPH oxidation in an atmosphere of 9:1 CO-O2. The other method relates the CO-sensitive drug oxidation to CO-sensitive NADPH-oxidation.
- Copyright ©, 1972, by Academic Press, Inc.
MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years.Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page.
|
Log in using your username and password
Purchase access
You may purchase access to this article. This will require you to create an account if you don't already have one.