Abstract
A potent, specific antiserum to rat liver phenylalanine hydroxylase was produced in sheep. Agar double-immunodiffusion reaction and enzyme inhibition studies revealed extensive cross-reactivity between the antiserum and phenylalanine hydroxylases from guinea pig, mouse, monkey, and human liver, as well as from rat kidney. No cross-reactivity with Pseudomonas phenylalanine hydroxylase could be demonstrated. Further studies suggested cross-reactivity with bovine adrenal and rat brain tyrosine hydroxylases, but not with rat brain tryptophan hydroxylase, rat kidney aromatic L-amino acid decarboxylase, or bovine adrenal dopamine β-hydroxylase.
- Copyright ©, 1972, by Academic Press, Inc.
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