WNT stimulation dissociates a Frizzled 4 inactive state complex with Gα12/13

Data Supplement

Files in this Data Supplement:

• Supplemental Figures -

  Supplemental Figure 1 - Functional validation of C terminally-tagged FZD4-GFP, FZD4- mCherry and FZD4-Cerulean in comparison to untagged FZD4 by DVL recruitment

  Supplemental Figure 2 - Functional validation of N terminally-tagged G12-mCherry employing p115-RHOGEF recruitment

  Supplemental Figure 3 - Functional validation of N terminally-tagged G13-mCherry employing p115-RHOGEF recruitment

  Supplemental Figure 4 - Quantification of LPA1 receptor-mediated p115-RHOGEF-GFP recruitment

  Supplemental Figure 5 - Expression levels of FZD4-GFP and forskolin-induced DMR responses in wt and Gα12/13-knock out HEK293 cells

  Supplemental Figure 6 - Expression levels of DVL1, 2, 3 in HEK293 cells treated with ctrl and panDVL siRNA