Targeting a PAR4 carboxyl terminal motif to regulate platelet function

Data Supplement

Files in this Data Supplement:

• Supplemental Data -

  Supplementary Figure 1 - AYPGKF-NH2 triggered calcium signaling in HEK cells transiently expressing wt-PAR4-YFP, QC-PAR4-YFP and dRS-PAR4-YFP

  Supplementary Figure 2 - (A) HEK cells expressing wt or dRS-PAR4 can activate ERK and P38 MAPK signaling in response to 100-mu-M AYPGKF-NH2 treatment for 10 minutes

  Supplementary Figure 3 - BRET detection of (A) Thrombin and (B) AYPGKF-NH2 triggered beta-arrestin recruitment to QC-PAR4

  Supplementary Figure 4 - BRET detection of beta-arrestin1/2 recruitment to PAR2 in the presence and absence of RAG8

• Supplementary Video 1 -

  FeCl3 triggered thrombosis in-vivo in SRQ8 treated control animals and RAG8 treated animals. Data are representative of three independent experiments