CYP26C1 is a hydroxylase of multiple active retinoids and interacts with cellular retinoic acid binding proteins

Data Supplement

• Supplemental Data -

  Supplemental Table 1 - Mass spectrometry parameters of MRM scans set for metabolite identification experiments
  

  Supplemental Table 2 - Mass spectrometry parameters of MRM scan used in enzyme kinetic experiments
  

  Supplemental Table 3 - Homology Model Quality Assessment
  

  Supplemental Table 4 - Amino acid residues assigned to secondary structure of CYP26C1 homology model
  

  Supplemental Table 5 - Proposed amino acid residues that interact with RA isomers
  

  Supplemental Figure 1 - Michaelis-Menten plots of metabolite formation
  

  Supplemental Figure 2 - RMSD (Panel A) and active site volume (Panel B) during MD simulations
  

  Supplemental Figure 3 - LC-UV chromatograms of CYP26C1 incubating with atROL (A), 13-cis-ROL (B), 9-cis-ROL (C)
  and 3-dehydroROL (D)
  

  Supplemental Figure 4 - MRM chromatogram and MS/MS spectra of atRA metabolites formed by CYP26C1
  

  Supplemental Figure 5 - MRM chromatogram and MS/MS spectra of 13-cis-RA metabolites formed by CYP26C1
  

  Supplemental Figure 6 - MRM chromatogram and MS/MS spectra of 9-cis-RA metabolites formed by CYP26C1