PT - JOURNAL ARTICLE AU - Jennifer L. Berkeley AU - Allan I. Levey TI - Cell-Specific Extracellular Signal-Regulated Kinase Activation by Multiple G Protein-Coupled Receptor Families in Hippocampus AID - 10.1124/mol.63.1.128 DP - 2003 Jan 01 TA - Molecular Pharmacology PG - 128--135 VI - 63 IP - 1 4099 - http://molpharm.aspetjournals.org/content/63/1/128.short 4100 - http://molpharm.aspetjournals.org/content/63/1/128.full SO - Mol Pharmacol2003 Jan 01; 63 AB - Several families of G protein-coupled receptors (GPCR) have been shown to activate extracellular signal-regulated kinase (ERK) in transfected cells and non-neuronal systems. However, little is known about GPCR activation of ERK in brain. Because ERK is an important component in the regulation of synaptic plasticity, in this study we examined ERK activation by three families of GPCR that respond to major neuromodulatory neurotransmitters in the hippocampus. We used an immunocytochemical approach to examine ERK activation by muscarinic acetylcholine (mAChR), metabotropic glutamate (mGluR), and β-adrenergic (β-AR) receptors in CA1 neurons of mouse hippocampal slices. Because these GPCR families comprise receptors coupling to each of the major heterotrimeric G proteins, we examined whether ERK activation differs according to G-protein coupling. By using immunocytochemistry, we were able to examine not only whether each family of receptors activates ERK, but also the cellular populations and subcellular distributions of activated ERK. We demonstrated that M1 mAChRs and group I mGluRs, both of which are Gq-coupled receptors, activate ERK in CA1 pyramidal neurons, although activation in response to mAChR is more robust. The Gi/o-coupled group II mGluRs activate ERK in glia scattered throughout CA1, and Gs-coupled β-AR receptors activate ERK in scattered interneurons. Thus, we demonstrated that GPCR coupling to Gq, Gi/o, and Gs all activate ERK in the hippocampus, although each does so with unique properties and distributions.