PT  - JOURNAL ARTICLE
AU  - JACQUELINE DOLAIS-KITABGI
AU  - ROBERT L. PERLMAN
TI  - The Stimulation of Cathecholamine Release from Chromaffin Granules by Valinomycin
DP  - 1975 Nov 01
TA  - Molecular Pharmacology
PG  - 745--750
VI  - 11
IP  - 6
4099  - http://molpharm.aspetjournals.org/content/11/6/745.short
4100  - http://molpharm.aspetjournals.org/content/11/6/745.full
SO  - Mol Pharmacol1975 Nov 01; 11
AB  - Valinomycin stimulates the release of catecholamine from isolated guinea pig adrenal medullary chromaffin granules incubated in vitro. Valinomycin-stimulated catecholamine release is dependent on the ionophore concentration and on the concentration of K+ in the incubation medium. In. 0.15 M KCl-5 mM Na·PO4, pH 7.0, valinomycin at 1 µM increases catecholamine release about 5-fold. The finding that catecholamine release is accompained by the release of adenine nucleotides and of dopamine β-hydroxylase from the chromaffin granules indicates that valinomycin induces the lysis of the granules. The effect of valinomycin on catecholamine release is also dependent upon the anions present in the incubation medium. The anions tested support valinomycin-stimulated catecholamine release in the order SCN-, I-, Br- > Cl- > acetate, F-, isethionate. These data suggest that, in the presence of a membrane-permeable anion, valinomycin-mediated transport of K+ into chromaffin granules leads to the osmotic lysis of these granules. ACKNOWLEDGMENT We are grateful to Ms. Michi Chen for technical assistance.