TY - JOUR T1 - Antiviral Activity of Poly(7-deazainosinic acid)-Derived Complexes <em>in Vitro</em> and <em>in Vivo</em> JF - Molecular Pharmacology JO - Mol Pharmacol SP - 1045 LP - 1051 VL - 12 IS - 6 AU - E. DE CLERCQ AU - V. G. EDY AU - P. F. TORRENCE AU - J. A. WATERS AU - B. WITKOP Y1 - 1976/11/01 UR - http://molpharm.aspetjournals.org/content/12/6/1045.abstract N2 - The antiviral activities and/or interferon-inducing abilities of poly(I)·poly(C), poly(I)·poly(br5C), poly(c7I)·poly(C), and poly(c7I)·poly(br5C) have been assessed in a variety of cell cultures (human, rabbit, and mouse) and animals (rabbits, mice). In cultured cells the compounds were also examined for inhibition of host cell macromolecule synthesis. Lethal effects were looked for in mice sensitized with lead acetate. Although the extent of antiviral activity of the polymers varied from one assay system to another, the following order of (decreasing) activity appeared applicable to all systems: poly(I)·poly(br5C) [unknown] poly(I)·poly(C) &gt; poly(c7I)·poly(b5C) [unknown] poly(c7I)·poly(C). Poly(I)·poly(C) and poly(I)·poly(br5C) did not differ markedly in antiviral activity, interferon-inducing ability, or toxicity. However, in human skin fibroblasts, poly(I)·poly(br5C) was distinctly superior as an interferon inducer, especially at lower doses. Poly(c7I)·poly(C) was the least active of the four polymers and was also the most potent inhibitor of host cell macromolecule synthesis. Poly(c7I)·poly(br5C) equaled or even surpassed poly(I)·poly(C) in interferon-inducing activity in human skin fibroblasts; in animals, however, it proved definitely less active. In determining the antiviral behavior of the complexes studied, two effector mechanisms should be taken into account: interferon induction and inhibition of macromolecule biosynthesis. Which of these parameters will predominate would ultimately depend on the fate of the double-stranded complex after it has reacted with the cell. ACKNOWLEDGMENTS The skillful technical assistance of Anita Van Lierde and Miette Stuyck and the editorial help of Janine Putzeys are gratefully acknowledged. The authors are indebted to Dr. Leonard Kohn, National Institutes of Health, for his help in determining sedimentation coefficients. ER -