RT Journal Article
SR Electronic
T1 The Relationship between Glucocorticoid Structure and Effects upon Thymocytes
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 948
OP 955
VO 13
IS 5
A1 J. P. DAUSSE
A1 D. DUVAL
A1 P. MEYER
A1 J. C. GAIGNAULT
A1 C. MARCHANDEAU
A1 J. P. RAYNAUD
YR 1977
UL http://molpharm.aspetjournals.org/content/13/5/948.abstract
AB Glucocorticoid hormones inhibit uridine incorporation into ribonucleic acid of thymocytes. The relationship between this effect and the binding to glucocorticoid receptors in mouse thymocytes was studied in parallel. The 27 different steroids examined could be classified as either agonists (e.g., dexamethasone, hydrocortisone, corticosterone), which bind to thymocytes and have an inhibitory effect on uridine incorporation; antagonists (e.g., 6α, 16α-dimethylprogesterone, progesterone), which bind to receptors without significant effect on uridine incorporation and which inhibit the biochemical effect of agonists; or inactive steroids (e.g., estradiol, cortisone), which neither bind nor have any effect on uridine incorporation. Combined evaluation in mouse thymocytes thus appears to be a useful tool in screening of steroids for glucocorticoid activity. ACKNOWLEDGMENTS The authors thank Mlle E. Goupy for typing the manuscript. They also thank Dr. J. W. Funder for helpful discussions and for critical reading of the manuscript.