TY - JOUR T1 - The Action of Halothane on Adenylate Cyclase JF - Molecular Pharmacology JO - Mol Pharmacol SP - 976 LP - 979 VL - 13 IS - 5 AU - LUBOS TRINER AU - YVONNE VULLIEMOZ AU - MARIAGNES VEROSKY Y1 - 1977/09/01 UR - http://molpharm.aspetjournals.org/content/13/5/976.abstract N2 - Halothane, at concentrations of from 1-10 volume %, significantly increased adenylate cyclase activity in rat uterine homogenates. The activation of adenylate cyclase by halothane was not altered by propranolol. The inhibitory effect of this beta adrenergic blocking compound on isoproterenol-stimulated adenylate cyclase was not changed by halothane, while the stimulation of adenylate cyclase by isoproterenol, including the maximally effective concentration, was enhanced. The activation of the enzyme by other agonists—prostaglandin E1, sodium fluoride, and 5'-guanylylimidodiphosphate—was also markedly increased by halothane at each concentration of the agonists tested, including maximally effective concentrations. It is proposed that halothane exerts its action on uterine adenylate cyclase through conformational changes of the catalytic unit, resulting in a higher activity of the enzyme. ACKNOWLEDGMENTS The authors thank Mrs. Olga Zak and Miss Gunilla Thulin for their excellent technical assistance. ER -