RT Journal Article
SR Electronic
T1 Synthesis of Methotrexate Polyglutamates in Cultured Human Cells
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 210
OP 214
VO 14
IS 1
A1 D. S. ROSENBLATT
A1 V. M. WHITEHEAD
A1 M. M. DUPONT
A1 M.-J. VUCHICH
A1 N. VERA
YR 1978
UL http://molpharm.aspetjournals.org/content/14/1/210.abstract
AB We describe the biosynthesis of po1y-γ-glutamyl metabolites of methotrexate in cultured human fibroblasts. The accumulation of polyglutamates by these cells is dependent on the concentration of methotrexate in the culture medium, duration of incubation, and stage of the culture cycle. When [3H]methotrexate is replaced with equimolar unlabeled methotrexate, label is found in successively longer polyglutamates. Synthesis of the polyglutamates is dependent on the continued presence of methotrexate in the culture medium.