RT Journal Article SR Electronic T1 Adenylate Cyclase in Cerebral Microvessels: Action of Guanine Nucleotides, Adenosine, and other Agonists JF Molecular Pharmacology JO Mol Pharmacol FD American Society for Pharmacology and Experimental Therapeutics SP 462 OP 472 VO 16 IS 2 A1 MINTA HUANG A1 GEORGE I. DRUMMOND YR 1979 UL http://molpharm.aspetjournals.org/content/16/2/462.abstract AB The isolation of microvessels (capillaries and arterioles) from guinea pig cerebral cortex is described. Adenylate cyclase in microvessel preparations was activated by guanylylimidodiphosphate in a time-dependent manner. The rate of guanyl nucleotide activation was enhanced by adenosine, 2-chloroadenosine, 2-azidoadenosine, norepinephrine, histamine, isoproterenol, prostaglandin E1, prostaglandin E2 and prostacyclin. Activation by adenosine analogues was demonstrable only when endogenously-generated adenosine was destroyed with adenosine deaminase. Activation was also in evidence when microvessels were incubated with agonists together with guanine nucleotide, then washed by sedimentation prior to assay, reflecting persistence of the activated state.