PT  - JOURNAL ARTICLE
AU  - SEDWICK, W. DAVID
AU  - FYFE, MARY JO
AU  - BROWN, OLIVER E.
AU  - FRAZER, THOMAS A.
AU  - KUTLER, MARC
AU  - LASZLO, JOHN
TI  - Deoxyuridine Incorporation as a Useful Measure of Methotrexate and Metoprine Uptake and Metabolic Effectiveness
DP  - 1979 Sep 01
TA  - Molecular Pharmacology
PG  - 607--613
VI  - 16
IP  - 2
4099  - http://molpharm.aspetjournals.org/content/16/2/607.short
4100  - http://molpharm.aspetjournals.org/content/16/2/607.full
SO  - Mol Pharmacol1979 Sep 01; 16
AB  - This paper contrasts the inhibition of deoxyuridine incorporation by the lipid-soluble antifolate, DDMP, and methotrexate in the human lymphoblastoid cell line, WIL-2. It shows how deoxyuridine incorporation can be used as an indirect tool for assay of drug uptake and facilitates interpretation of experiments with labeled drugs. Whereas 1 µM methotrexate takes 15-30 minutes to maximally inhibit deoxyuridine incorporation, DDMP maximally inhibits incorporation within five seconds of addition to the cell culture. These protocols expand the potential of deoxyuridine-based assays in analysis of antifolate action and may be useful for in vitro testing of potential tumor responses to antifolates.