PT  - JOURNAL ARTICLE
AU  - BAKSI, SAMARENDRA N.
AU  - KENNY, ALEXANDER D.
TI  - Parathyroid Hormone Stimulation of 1,25-Dihydroxyvitamin D<sub>3</sub> Production in Antiestrogen-treated Japanese Quail
DP  - 1979 Nov 01
TA  - Molecular Pharmacology
PG  - 932--940
VI  - 16
IP  - 3
4099  - http://molpharm.aspetjournals.org/content/16/3/932.short
4100  - http://molpharm.aspetjournals.org/content/16/3/932.full
SO  - Mol Pharmacol1979 Nov 01; 16
AB  - In vivo administration of estradiol benzoate (E2B) or parathyroid hormone (PTH) stimulates in vitro renal conversion of 25-hydroxyvitamin D3 [25-(OH)D3] to 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] in birds. In the present investigation, the effect of PTH on 1,25-(OH)2D3 production was studied in female Japanese quail pretreated with the antiestrogen, tamoxifen citrate (30 mg/kg) daily for 7 days. PTH was injected at two different doses (92 and 275 U.S.P. units/kg) every 8 hours during the last two days of tamoxifen treatment. E2B (0.1 and 1.0 mg/kg) was injected 24 hours before sacrifice. Control groups received vehicle only. All injections were given intramuscularly. Kidney homogenates were prepared and incubated with tritiated 25-(OH)D3. E2B-induced stimulation of 1,25-(OH)2D3 production was completely blocked by prior treatment with tamoxifen, but PTH-induced stimulation of 1,25-(OH)2D3 production was unaffected by antiestrogen treatment. When PTH and E2B were injected together, no additive effects on either 1,25-(OH)2D3 production or hypercalcemia were observed. The data indicate that estrogen receptors are not an essential link in the chain of events mediating the PTH-induced stimulation of 1,25-(OH)2D3 production. ACKNOWLEDGMENTS The authors are grateful for the technical assistance of Stephen M. Fuller, Vicki D. Edgington and Ingrid L. Greene.