RT Journal Article
SR Electronic
T1 Retinal Tyrosine Hydroxylase: Comparison of Short-term and Long-term Stimulation by Light
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 1212
OP 1219
VO 14
IS 6
A1 P. MICHAEL IUVONE
A1 C. L. GALLI
A1 N. H. NEFF
YR 1978
UL http://molpharm.aspetjournals.org/content/14/6/1212.abstract
AB Dopamime (DA) is a putative neurotransmitter within some retinal amacrine neurons. Exposure of rats to light for either 15 min or 96 hr increases retinal tyrosine hydroxylase activity. Concomitant with the increase of enzyme activity is a 4-fold increase of DA formation. The molecular mechanism for the increased enzyme activity for the two exposures to light is apparently different. Short-term exposure to light decreases the Km but not the Vmax of the enzyme for the pteridine cofactor, while 96 hr of exposure to light increases the Vmax for tyrosine but has no significant effect on the Km for tyrosine or cofactor. Enzyme activity of rats exposed to 15 min of light decreases to the level found in 96 hr dark-adapted rats within 30 min of darkness, while more than 6 hr of darkness are required after 96 hr of light exposure. Our studies are consistant with the hypothesis that retinal DA formation is modulated by different molecular mechanisms depending on the duration of exposure of light. Short-term exposure to light activates tyrosine hydroxylase while long-term exposure to light results in the formation of more active molecules of enzyme.