PT  - JOURNAL ARTICLE
AU  - AWNI M. SARRIF
AU  - HIROSHI TONE
AU  - PETER V. DANENBERG
AU  - CHARLES HEIDELBERGER
TI  - The Incorporation of Trifluorothymidine into Calf Thymus DNA in a Cell-Free System Does Not Lead to Chain Termination
DP  - 1980 Jul 01
TA  - Molecular Pharmacology
PG  - 148--150
VI  - 18
IP  - 1
4099  - http://molpharm.aspetjournals.org/content/18/1/148.short
4100  - http://molpharm.aspetjournals.org/content/18/1/148.full
SO  - Mol Pharmacol1980 Jul 01; 18
AB  - In this report we present evidence that trifluorothymidine-5'-triphosphate (F3dTTP) once incorporated into activated calf thymus DNA by a partially purified HeLa cell cytoplasmic DNA-dependent DNA polymerase α does not lead to chain termination. Less than 1% of the [3H]F3dTTP incorporated in the cell-free system was recovered at the 3'-termini. In addition, F3dTTP-containing DNA was capable of incorporating [3H]dTTP, which led to the complete disappearance of radioactive trifluorothymidine from the 3'-termini. ACKNOWLEDGMENTS The authors acknowledge Ms. Virginia Garcia for her technical assistance and Mrs. Sharon Garrison for the preparation of the manuscript.