RT Journal Article
SR Electronic
T1 Characterization of Serotonin Uptake in Isolated Bovine Pinealocyte Suspensions
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 447
OP 454
VO 18
IS 3
A1 DUCIS, ILZE
A1 DISTEFANO, VICTOR
YR 1980
UL http://molpharm.aspetjournals.org/content/18/3/447.abstract
AB The uptake of radiolabeled serotonin as a function of concentration exhibited saturation when low (0.005-2 µM) concentrations of serotonin were used. Kinetics of the initial rate (30 s) of entry of radiolabeled serotonin into pinealocytes were examined using various concentrations of the substrate. The effects of temperature, changes in external ion concentrations, drugs, metabolic inhibitors, and other compounds on the uptake of serotonin were investigated. High concentrations (10 µM-5 mM) of serotonin appear to use amino acid transport systems, e.g., L-lysine, L-leucine, and L-alanine. Uptake is partially sodium dependent, temperature dependent, sensitive to metabolic inhibitors, and inhibited by various amino acids. Low concentrations (0.005-1 µM) of serotonin appear to use a highly specific, allosteric carrier for transport. Uptake is sodium dependent, temperature dependent, and sensitive to a variety of metabolic inhibitors.